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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (12): 331-331.

• 植物保护 农药 • 上一篇    下一篇

白花曼陀罗叶总碱提取物杀线活性及其作用机理研究

陆秀红,刘志明,,刘纪霜,黄金玲,潘萍红   

  • 出版日期:2006-12-05 发布日期:2006-12-05

Nematicidal Activities and Mechanisms of Total Alkaloids Extracts from Leaf of Datura metel Linn.

Lu Xiuhong, Liu Zhiming, Huang Jinling, Liu Jishuang   

  • Online:2006-12-05 Published:2006-12-05

摘要： 为了解白花曼陀罗的杀线活性物质及其作用机理，对白花曼陀罗叶的总生物碱进行了提取鉴定，并通过测定乙酰胆碱酯酶活性及乙酰胆碱含量研究其作用机理，结果表明：生物碱是白花曼陀罗的重要杀线活性物质，可抑制线虫乙酰胆碱酯酶活性，提高线虫乙酰胆碱含量，直接作用于线虫的神经系统。

关键词: 小金海棠, 小金海棠, 抑制性消减杂交, cDNA文库

Abstract: In order to know the nemsticifal activities substance and the mechanisms of Datura metel Linn, total alkaloids from the leaf of Datura metel Linn was extracted and identified, and studied the mechanism of it by determining the activity of Ache and the Ach content of Meloidogyne incognita. The results showed that alkaloids played a determinant role in nematocidal activity. It suppressed the activity of AchE , increased the number of Ach, could attack the nematode neural system.

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