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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 105-110.doi: 10.11924/j.issn.1000-6850.2014-1409

所属专题： 生物技术； 园艺

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热胁迫下茄子叶片cDNA-SSH文库的构建及其相关基因分析

杨洋,田时炳,王永清,罗章勇,王之劲,谢树章   

  1. 重庆市农业科学院,重庆市农科院蔬菜花卉所,重庆市农业科学院蔬菜花卉所,重庆市农业科学院蔬菜花卉所,重庆市农科院蔬菜花卉所,重庆市农业科学院生物技术研究中心
  • 收稿日期:2014-05-15 修回日期:2014-05-15 接受日期:2014-09-05 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 杨洋

Analysis of Genes Related to Heat Stress in Leaves of Eggplant SSH-cDNA Libraries

  • Received:2014-05-15 Revised:2014-05-15 Accepted:2014-09-05 Online:2014-10-15 Published:2014-10-15

摘要： 通过获得热胁迫下茄子热应激表达功能基因，可为创制茄子耐热性品种提供研究基础。笔者采用抑制性消减杂交的方法构建了茄子耐热品系896 的热胁迫基因上调表达文库，并对文库的部分序列进行了实时荧光定量PCR表达验证分析。构建文库信息分析表明，茄子受热胁迫后大量因子参与到代谢过程的调控中，主要参与糖类、脂类、能量及氨基酸代谢途径，体内的激素调控表现为生长抑制调控。获得文库中编码小分子热激蛋白的YP16，编码碳酸酐酶的YP61 以及编码钙依赖蛋白激酶的YP248 在茄子叶片受热胁迫后均上调表达。

关键词: 调控, 调控

Abstract: Analysis of genes related to heat stress of eggplant can provide basis for cultivating heat tolerant varieties of eggplant. A SSH-cDNA library of heat-shocked eggplant 896 was constructed by suppression subtractive hybridization method. Real- time quantitative PCR expression analysis further validated the expression modes of partial sequences in this library. Library analysis showed that a large number of unigenes participated in the regulation of eggplant metabolic processes, including carbohydrate, lipid, energy and amino acid metabolic pathways, hormonal regulation of the heat-shocked eggplant expressed as growth inhibition in vivo. YP16 putative encoding a small heat shock protein, YP61 putative encoding carbonic anhydrase and YP248 putative encoding calcium-dependent protein kinase were confirmed to have up-regulated expression in eggplant leaves after the heat-shock.