鱼组织中氟喹诺酮类药的HPCE多残留检测方法的建立

摘要/Abstract

摘要： 建立了以伊诺沙星为内标，环丙沙星、恩诺沙星、沙拉沙星、二氟沙星在鱼组织中的毛细管电泳多残留检测方法。研究了缓冲溶液的类型，pH值，有机试剂添加等电泳条件.得到最佳的缓冲溶液是含10 mL/L乙腈的pH 5.0的磷酸氢二钠-柠檬酸缓冲溶液。用C18固相萃取柱浓缩和净化处理,用二极管阵列检测器进行检测(275 nm)。结果，四种药物在组织中的溶度25~400 μg/kg范围内R＞0.999,线性关系良好。环丙沙星，恩诺沙星，沙拉沙星和二氟沙星回收率分别为75.2%,79.7%，80.1%，79.2%。恩诺沙星的检测限低于25 μg/kg，其余的检测限均为25 μg/kg,，方法的日内变异系数小于13.5%，日间变异系数小于12.3%。该方法简单快速，经济环保，适用于大量阳性样本的筛选。

关键词: 干细胞, 干细胞, 肿瘤发生, 肿瘤治疗

Abstract: A method for the determination of veterinary antimicrobial agent enrofloxacin, ciprofloxacin, sarafloxacin and difloxacin in fish muscle, using enoxacin as internal standard, has been developed. The influence of different separation conditions, such as the buffer, pH of the electrolyte and the organic added to the electrolyte was studied. The most suitable buffer is Na2HPO4-citrate at pH 5.0 containing 10%(v/v) acetonitrile. Clean-up and pre-concentration of the samples were effected by C18 solid-phase extraction and determination was carried out by capillary electrophoresis using a photodiode array detector (275nm). The calibration graphs are linear for the four fluoroquinolones from 25 to 400 μg/kg. The method recoveries for enrofloxacin、ciprofloxacin、sarafloxacin and difloxacin are 75.2%、79.7%、80.1%、79.2%. The limit of detection for Enrofloxacin is lower than 25 μg/kg, and the other are 25 μg/kg. The Rsds for intraday and interday are not more than 13.5% and 12.3%, respectively. It is a fast and simple method that allows the detection of positive muscle samples.

饶钦雄,刘慧慧,刘向明,沈建忠,,江海洋,,丁双阳,. 鱼组织中氟喹诺酮类药的HPCE多残留检测方法的建立[J]. 中国农学通报, 2007, 23(2): 93-093.

Rao Qinxiong, Liu Huihui, Liu Xiangming, Shen Jianzhong，, Jiang Haiyang，, Ding Shuangyang，. Determination of Fluoroquinolone Residues in Fish Muscle by Capillary Electrophoresis[J]. Chinese Agricultural Science Bulletin, 2007, 23(2): 93-093.

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