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中国农学通报 ›› 2007, Vol. 23 ›› Issue (2): 89-089.

所属专题: 生物技术

• 目次 • 上一篇    下一篇

亚洲百合DNA的提取及RAPD-PCR反应体系的优化

赵 琛   

  • 出版日期:2007-02-05 发布日期:2007-02-05

DNA Extraction and Optimization of RAPD Reaction System for Lilium Asiatic hybrids (Liliaceae)

Zhao Chen   

  • Online:2007-02-05 Published:2007-02-05

摘要: 为了促进亚洲百合分子水平的研究,以亚洲百合幼嫩叶片为材料,采用改良CTAB法提取亚洲百合基因组DNA,得到的DNA质量较高,适合于RAPD -PCR分析。通过单因素优化得到在25μl反应体系中,亚洲百合RAPD 分析的最佳反应体系:160μmol/ L dNTPs,0.3μmol/L随机引物,Taq DNA聚合酶1.5U,Mg2+浓度2.0mmol/L。

关键词: 马铃薯, 马铃薯, 生物技术, 品质, 性状

Abstract: In order to accelerate the molecular biology research of Lilium asiatic hybrids, DNA of Lilium asiatic hybrids was extracted from the fresh leaves by CTAB. The optimized reaction system for Lilium asiatic hybrids are as follows. The reactions were performed in a volume of 25 μl containing 2.0 mmol/L Mg2+, 160 μmol/L dNTPs, 1.5 U Taq DNApolymerase, 0.3 μmol/L primer.

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