应用剩余杂合体衍生的近等基因系定位水稻株高QTL

doi:10.11924/j.issn.1000-6850.casb2021-0510

中国农学通报 ›› 2022, Vol. 38 ›› Issue (9): 1-5.doi: 10.11924/j.issn.1000-6850.casb2021-0510

所属专题：生物技术；水稻

• 农学·农业基础科学 • 下一篇

应用剩余杂合体衍生的近等基因系定位水稻株高QTL

罗先富(), 刘文强, 潘孝武, 董铮, 刘三雄, 刘利成, 阳标仁, 盛新年, 李小湘()

湖南省农业科学院水稻研究所/农业部长江中下游籼稻遗传育种重点实验室,长沙 410125

收稿日期:2021-05-14 修回日期:2021-08-13 出版日期:2022-03-25 发布日期:2022-04-02
通讯作者: 李小湘
作者简介:罗先富,男,1975年生,湖南安乡人,研究员,学士,研究方向：水稻遗传育种。通信地址：410125 湖南省长沙市芙蓉区马坡岭远大二路湖南省水稻研究所932号,Tel：0731-84691584,E-mail： 1153677355@qq.com。
基金资助:
长沙市重点研发计划项目“水稻种子活力相关QTL的精细定位、克隆及育种价值分析”(kq2004045);湖南省农业科学院科技创新项目“基于MAGIC群体的水稻早花性状全基因组关联分析研究”(2020CK17)

Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice

LUO Xianfu(), LIU Wenqiang, PAN Xiaowu, DONG Zheng, LIU Sanxiong, LIU Licheng, YANG Biaoren, SHENG Xinnian, LI Xiaoxiang()

Rice Research Institute, Hunan Academy of Agricultural Science/Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of the Yangtze River Valley, Ministry of Agriculture and Rural Affairs, Changsha 410125

Received:2021-05-14 Revised:2021-08-13 Online:2022-03-25 Published:2022-04-02
Contact: LI Xiaoxiang

摘要/Abstract

摘要：

为挖掘有育种利用价值的水稻株高新基因,以来源于‘Katy’/‘湘743’且在第1染色体RM11383-RM1198区间杂合的一个剩余杂合体RHL1030 (F₁₀)为材料,遗传分析RHL1030衍生群体,显示在该区间鉴定到控制株高QTL,增效等位基因来自于‘湘743’。应用SSR标记检测,从RHL1030衍生群体筛选杂合区间分别为RM3411-RM11782和RM6703-RM1198的两个单株,自交一代形成2个F₂群体,验证并界定株高QTL在RM6703-RM1198区间。从RM6703-RM1198区间分离群体筛选RM6703-RM8085区间杂合的3个单株和RM5389-RM1198区间杂合的1个单株,自交一代形成4个F₂群体,从群体中分别筛选母本纯合型、父本纯合型和杂合型单株各40株构成近等基因系进行方差分析,最终界定株高QTL在RM11782-RM5389区间,物理位置34.17M~35.73 Mb。本研究定位到一个新的控制株高QTL,为改良水稻株型提供资源。

关键词: 剩余杂合体, 近等基因系, 株高, 水稻, 数量性状位点

Abstract:

To explore a novel and valuable gene for plant height in rice breeding, a residual heterozygous line (RHL) RHL1030 (F₁₀) derived from a cross between ‘Katy’ and ‘Xiang743’, with a heterozygous interval of RM11383-RM1198 on chromosome 1 was used as the genetic material. Genetic analysis of RHL1030-derived population showed that a QTL for plant height was identified in the interval, the additive-effect QTL came from ‘Xiang743’. Two individual plants respectively with heterozygous intervals of RM3411-RM11782 and RM6703-RM1198 were selected from the RHL1030-derived population on the basis of SSR genotypes, and self-pollinated to develop two F₂ populations. The QTL was validated and further delimited in the interval of RM6703-RM1198. Three individual plants with heterozygous intervals of RM6703-RM8085 and one individual plant with a heterozygous interval of RM5389-RM1198 were selected from the population derived from a RHL with a heterozygous interval of RM6703-RM1198, and self-pollinated to develop four resultant F₂ populations. Forty maternal homozygotes, paternal homozygotes and heterozygotes respectively were selected from the four F₂ populations to develop near isogenic lines for ANOVA analysis. The QTL was finally delimited in the interval of RM11782-RM5389, and the corresponding physical position was from 34.17 Mb to 35.73 Mb. This study identifies a novel QTL for plant height, and provides a resource for improving plant architecture in rice.

Key words: residual heterozygous line (RHL), near isogenic line (NIL), plant height, rice (Oryza sativa L.), quantitative trait locus (QTL)

中图分类号:

S184

罗先富, 刘文强, 潘孝武, 董铮, 刘三雄, 刘利成, 阳标仁, 盛新年, 李小湘. 应用剩余杂合体衍生的近等基因系定位水稻株高QTL[J]. 中国农学通报, 2022, 38(9): 1-5.

LUO Xianfu, LIU Wenqiang, PAN Xiaowu, DONG Zheng, LIU Sanxiong, LIU Licheng, YANG Biaoren, SHENG Xinnian, LI Xiaoxiang. Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice[J]. Chinese Agricultural Science Bulletin, 2022, 38(9): 1-5.

图/表 3

参考文献 20

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群体	杂合区间	基因型值¹			F	P	加性效应	显性效应	R²/%²
XK2-5	RM6703-RM8085	158.3	126.6	146.8	50.7	<0.000001	15.9	4.35	63.1
XK10-7	RM6703-RM8085	163.6	127.5	152.2	15.9	<0.000001	18.1	6.65	69.2
XK13-1	RM6703-RM8085	168.9	135.4	156.8	41.2	<0.000001	16.8	4.65	74.1
XK5-4	RM5389-RM1198	120.3	118.6	117.3	1.3	0.26

群体	杂合区间	基因型值¹			F	P	加性效应	显性效应	R²/%²
XK2-5	RM6703-RM8085	158.3	126.6	146.8	50.7	<0.000001	15.9	4.35	63.1
XK10-7	RM6703-RM8085	163.6	127.5	152.2	15.9	<0.000001	18.1	6.65	69.2
XK13-1	RM6703-RM8085	168.9	135.4	156.8	41.2	<0.000001	16.8	4.65	74.1
XK5-4	RM5389-RM1198	120.3	118.6	117.3	1.3	0.26

应用剩余杂合体衍生的近等基因系定位水稻株高QTL

Mapping of Plant Height QTL Using NILs Derived from Residual Heterozygous Lines in Rice

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