Abstract: 【Objective】 A Silver-Enhanced Colloidal Gold Assay was developed for detection on anti-IBV antibody in chicken. 【Method】 The purified rabbit anti-chicken IgG was labeled with colloidal gold of 10nm in diameter. Then purified IBV was coated onto NCM with 0.4μg each dot. After being blocked, the NCM was immersed in serum samples diluted with different titer (10×2x) for 10 minutes and then reacted in solution of rabbit anti-chicken IgG labeled with colloidal gold diluted with 1:4 for 1 hour. In the end NCM was stained 10 minutes by silver and the result was observed. 【Results】 IBV positive serum titer was more than 10×27, whereas ND, IBD, EDS-76 positive serum titer was less than 10×22, which indicated cross-reaction had no signification. The detection limit of chicken IgG was 0.88ng. SECGA, Serum Neutralization in TOCS and ELISA were significantly co-relative when anti-IBV antibody was detected using them respectively. 【Conclusion】 These results indicated that SECGA could be used for antibody surveillance of IB. At the same time the assay does not need specially experimental instruments, and the detected results are objective and apt to judge with the naked eye. Therefore the method is suitable for using widely in the basic farms and firms.