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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (26): 14-18.doi: 10.11924/j.issn.1000-6850.2013-0123

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸡传染性法氏囊病病毒的分离鉴定与培养特性研究

常婧竹 李燕 周云飞 李新生 高文明 崔保安   

  • 收稿日期:2013-01-11 修回日期:2013-02-04 出版日期:2013-09-15 发布日期:2013-09-15
  • 基金资助:
    鸡主要疫病综合免疫技术中试与示范

Study on Isolation, Identification and Cultivate Characteristics for Chicken Infectious Bursal Disease Virus

  • Received:2013-01-11 Revised:2013-02-04 Online:2013-09-15 Published:2013-09-15

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摘要： 为研究鸡传染性法氏囊病病毒在不同宿主上的培养特性，从河南省某疑似感染鸡传染性法氏囊病病毒的鸡场采集病料，通过琼脂免疫扩散试验初步筛选出IBDV毒株，并将毒株回归易感鸡，在SPF鸡胚及DF1细胞上培养。结果显示：培养出的病毒作为抗原与标准阳性血清之间均出现阳性沉淀线；回归SPF鸡试验显示发病率达100%，死亡达60%，病死鸡只出现法氏囊肿大、出血甚至出现紫葡萄样；该毒株能引起鸡胚CAM增厚、胚体出血，引起DF1细胞变圆脱落；ELD50与TCID50分别为10-4.6/0.1 mL、10-5.5/0.1 mL。试验证实IBDV分离株，属于超强毒株，该毒株可以在鸡胚和DF1细胞上适应增殖，将其命名为HN12号分离株。

关键词: 生理特性, 生理特性

Abstract: To study the cultivate characteristics in different host, An IBDV strain was selected preliminary with Agar diffusion test, which collected from a chicken farm suspected infection with infectious bursal disease virus in Henan Province. The strain were infected susceptibility chicken, SPF chicken and DF1 cells. The results showed that, there were positive precipitation line in AGID; This strain could cause SPF chickens (morbidity with 100% and mortality with 60%) bursal swelling, bleeding, and even purple grape-like were showded; cause chick embryo CAM thickening, chicken embryos bleeding, cause the DF1 cells round off, the ELD50 and TCID50 was respectively 10-4.6/0.1 mL and 10-5.5/0.1 mL. The results confirmed that the isolated IBDV stain belongs to vvIBDV, it could replicate in chicken embryo and cell. It was named HN12.

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