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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (26): 9-13.doi: 10.11924/j.issn.1000-6850.2013-0185

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

母源性印记基因在牛早期胚胎中的表达

赵德超 张春强 张文广 张家新   

  • 收稿日期:2013-01-18 修回日期:2013-03-14 出版日期:2013-09-15 发布日期:2013-09-15
  • 基金资助:
    国家科技计划；内蒙古自然科学基金资助。

The Expression of Maternal Imprinting Gene in the Bovine Early Stage Embryos

  • Received:2013-01-18 Revised:2013-03-14 Online:2013-09-15 Published:2013-09-15

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摘要： 为研究母源性印记基因Magel2、Sgce、SNRPN在牛早期胚胎中的表达规律，初步判定其印记发生的阶段。利用Real-time PCR技术，检测了这3个基因在牛孤雌激活 (Parthenogenetic Activation，PA) 胚胎和体外受精(In vitro Fertilization，IVF)胚胎中的表达水平。结果表明：在牛IVF和PA 2种胚胎间，3个母源性印记基因的印记表达模式明显不同，Magel2在牛IVF胚胎各阶段都有表达，而在PA胚胎中没有检测到表达信号；Sgce在牛IVF胚胎各阶段都有表达，而只在PA胚胎的2~4细胞阶段检测到表达，在其他发育阶段均没有表达；SNRPN在牛IVF胚胎各阶段都有表达，而只在PA胚胎的2细胞和囊胚阶段检测到很微弱的表达。实验结果表明，母源性印记基因Magel2、Sgce、SNRPN的正常表达对牛早期胚胎发育具有重要作用。

关键词: 筛选, 筛选

Abstract: In order to detect the expression profiles of the maternal imprinted genes Magel2, Sgce and SNRPN in the earlier bovine embryos, as well as preliminary determine the stage from which the imprinting start. In this study, the expression profiles of three maternal imprinted genes were detected between bovine Parthenogenetic Activation (PA) and in vitro Fertilization (IVF) embryos by Real-time PCR. The results showed that the expression patterns of the three genes were significantly different between IVF and PA embryos. Magel2 was expressed from 2 to blastocyst in bovine IVF embryos, while no detectable transcripts were observed in PA embryos. Sgce was expressed from 2 to blastocyst in bovine IVF embryos, while 2 to 4 cell stage in PA embryos. In bovine IVF embryos SNRPN transcripts could be detected from 2 to blastocyst stage, but it was only detected in 2-4 cell stage PA embryo.SNRPN expression could be detected in bovine IVF embryos from 2 cell to blastocyst stages, meanwhile it was only detected in PA 2 cell and blastocyst stage embryos. The results showed that maternal imprint genes Magel2, Sgce and SNRPN may might exert important roles on bovine early embryonic development.