关键词: 狗舌草, 狗舌草, 淋巴性白血病, L1210细胞, DBA/2小鼠

Abstract: The present study utilized mature seeds of wheat (Triticum aestivum L.) cultivar mianyang11 as the target material cultured in vitro, and the plasmid PBI121 which contains the cycd2 cell cycle protein gene and selectable marker NptII gene had been transferred into callus initiated from mature embryo of wheat by micro-projectile bombardment. The result showed that some transformation wheat could be refrained effectively and regenerated from the selection medium containing G418 solution. Furthermore, four plants were identified to be the transgenic ones containing the target genes of CycD2 by PCR and Southern-blot analysis.