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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (7): 46-46.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

犬淋巴细胞转化增殖试验最佳条件的确定

孔庆波,,刘剑郁,常建军,陈德坤   

  • 出版日期:2007-07-05 发布日期:2007-07-05

The Test Condition on the Test of Canine Lymphocytes Proliferation

Kong Qingbo,, Liu Jianyu, Chang Jianjun,Chen Dekun   

  • Online:2007-07-05 Published:2007-07-05

摘要： 本试验选择杂种牧羊犬作为实验犬，用MTT法检探讨了不同浓度犊牛血清（FCS）、淋巴细胞、植物血凝素P（PHA-P）和刀豆蛋白A（ConA）对犬淋巴细胞增殖的影响。确定了犊牛血清含量为10%，淋巴细胞浓度为5×106个/mL，用丝裂原PHA-P 刺激的最佳浓度为12.5ug/mL时，MTT法测定能够刺激犬淋巴细胞转化增殖，但t检验分析表明其促进淋巴细胞增殖的效果并不显著；当其它条件不变，所用的丝裂原为Con A时，它在浓度为0.5～2.5μg/mL范围内均可以显著刺激T细胞的增殖（P<0.05），且在浓度为2.5μg/mL时刺激效果最佳

关键词: 基因组文库, 基因组文库, 西农萨能奶山羊

Abstract: In this present test, a range of hybrid shepherd dogs were selected firstly. Afterwards, the effects of FCS，PBMC，PHA-P of different concentration on promote lymphocytes proliferation were tested by MTT test. The result of experiment demonstrate that the best levels combination of canine lymphocyte，calf blood serum were 5×106 unit/ml， 10% respectively. In the lymphocytic transfer test, PHA-P at dosage of 12.5μg/ ml performed facilitation effects for canine lymphocytic proliferation (p>0.05). ConA at 0.5~2.5μg/ mL markedly proliferated T lymphocytes (p<0.05), with the best effect at 2.5μg/ ml.

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