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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (4): 33-33.

所属专题： 生物技术； 马铃薯

• 目次 • 上一篇    下一篇

甘薯茎尖分生组织培养与快速繁殖技术研究※?

关崇梅,秦静远,徐志英,刘玉凤,张西丽,龙金娟   

  • 出版日期:2004-08-05 发布日期:2004-08-05

Research on Stem-apex Meristem Culture and Fast Reproduction Technology of Sweet Potato

GUAN Chong-mei, QIN Jin-yuan, XU Zhi-ying, LIU Yu-feng, ZHANG Xi-li, LONG Jin-juan   

  • Online:2004-08-05 Published:2004-08-05

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摘要： 甘薯茎尖分生组织培养与快速繁殖技术研究课题己进行3年,筛选出最佳培养基为MS+6-BA1.0mg/L+NAA0.1～0.2mg/L＋3%蔗糖＋琼脂6.0g/L；最适宜培养温度为26～30℃；症状学诊断法、指示植物鉴定法进行病毒检测，平均脱毒率达83.7%。脱毒试管苗切段快速增殖，繁殖系数为5n，繁殖速度以几何级数增长，一定时间内可大量繁殖脱毒苗。脱毒苗移栽大田进行产量比较，平均增产率达37.8％。

Abstract: The three-year research on stem-apex meristem culture and fast reproduction technology of sweet potato selected out MS+6-BA 1.0mg/L+NAA 0.1～0.2mg/L＋3%sucrose＋agar6.0g/L and 26～30℃ respectively as the preferential medium and appropriate temperature range. And the average devirus rate was 83.7% based on the semiological diagnostic and plant indicator identification. The reproduction coefficient for the fast propagation with cut test-tube plantlets was 5 n, the reproduction speed increased at a geometric progression and large-scale seedlings could be multiplied in a short time. The virus-free seedlings transplanted in fields reached a yield increase of 37.8%.