欢迎访问《中国农学通报》,

中国农学通报 ›› 2003, Vol. 19 ›› Issue (6): 16-16.

• 目次 • 上一篇    下一篇

高粱空间诱变效应研究

龚振平,刘自华,刘根齐 ,孟庆祥 ,刘振兴   

  • 出版日期:2003-12-25 发布日期:2003-12-25

Studies on Mutagenic Effect of High Space on Sorghum

GONG Zhen-ping, LIU Zi-hua, LIU Gen-qi, MENG Qing-xiang, LIU Zhen-xing   

  • Online:2003-12-25 Published:2003-12-25

摘要: 1996年利用我国第17颗返回式卫星对高粱唐恢28恢复系种子进行搭载处理后,对其变异后代进行了田间鉴定和同工酶及RAPD分析。主要结果如下:(1)SP1代获得一个特大穗型突变体(MTR28),其后代性状分离广泛,变异类型丰富,从中获得了遗传性状稳定且具有不同特点的高产、抗病优良变异选系;(2)优良变异选系组配的杂交种产量明显高于对照,可应用于生产上组配高产、抗病杂交种;(3)空间诱变后代性状稳定时间比常规杂交后代稳定快2—4个世代,可缩短育种进程;(4)变异选系在酯酶同工酶和细胞色素氧化酶同工酶酶带种类及酶活性上存在着较大的遗传差异;(5)RAPD分析结果显示,空间诱变选系在基因组水平上发生了明显的变化。

Abstract: RAPD analysis for mutated seeds of Tanghui-28 generations, which were returned by No.17 returning satellite,plus its field inspection and isozyme, has been carried out. The results were as follows: (1) a biggest mutated individual which was characterized by selected generations’ rich varieties, stable genetic traits, high yielding as well as disease resistance. (2) Excellent selected lines, which combinations’ yield was much bigger than that of contrast, could be used to make high yielding and disease resistance cross a parental lines. (3) There were 2~4 generations earlier ordinary ,that was also to say ,high space mutation could make the breeding programme develop in advance. (4) Their were signifficant genetic differerces among the mutated generations and its selected generations in some areas, such as esterase isozyme,cytochrome exidase isozyme as well as isozyme vigor. (5) RAPD analysis showed that selected lines by space mutation method varied signifficantly in DNA level.