Abstract: DUS testing guidelines of Hevea Brasilensis were established which based on basic requirements of International Union for the Protection of New Varieties of Plants (UPOV) and biological characters for enhancing the protection of new variety. The formulation process, testing particularities , rules of character selection and the development direction of DUS testing were introduced in this paper. It was suggested that the criterions of DUS testing should not be so high, and the testing characters should demonstrate Chinese breeding level and direction by following the UPOV principles. It was believed that techniques combined molecular markers and morphology testing will become the primary means of DUS testing on rubber.