Abstract: The mitochondrial DNA of Chinese cabbage CMS line and its maintainer line were analysed by the method of random amplified polymorphic DNA using random primers. And obtained clear amplification profile. Meanwhile, some obvious differences in mitochondrial genome were detected between CMS and maintainer line.And obtained specific amplified fragment (mt)S259~600bp of CMS in mitochondria DNA, the results revealed that and there are 23bp homology between (mt)S259~600bp and rape mitochondrion gene for NADH dehydrogenase subunit 4. The relationship of this different bands in CMS to male sterility needs further study.