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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 71-74.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

正交设计在彩色马蹄莲组织培养中的应用

张军云 杨向红 李恒仁 王文智 王文智 王文智 王文智   

  • 收稿日期:2008-07-23 修回日期:2010-06-01 出版日期:2010-08-05 发布日期:2010-08-05
  • 基金资助:

    清远市科技课题070002，清远职业技术学院资助课题KY070004

Application in Tissue Culture of the Colored Common Calla of Orthogonal Design

  • Received:2008-07-23 Revised:2010-06-01 Online:2010-08-05 Published:2010-08-05

摘要：

本试验通过正交设计方法，着重研究彩色马蹄莲组织培养过程中的基本培养基及植物生长调节剂6-BA、KT、IAA、NAA，对不定芽诱导、继代培养和诱导生根的影响。结果显示：初代培养BA2.0mg/L和B5培养基搭配效果最好，继代培养最优组合B5＋BA2.0mg/L+NAA0.1mg/L，诱导生根1/2MS＋NAA0.1mg/L+IBA0.3mg/L对生根率的诱导效果最好。

Abstract:

This trial is by way of the orthogonal design method, and stresses to study colored horse's hoof lotus organizations to cultivate basic culture medium and plant growth regulator 6-BA in the course and KT, IAA and NAA, guide to the adventitious bud and continues generation cultivating and guides the influence taking root. The result shows: it is the best just generation to cultivate BA2.0mg/L and B5's culture medium collocation effect, and continues era cultivating the most excellent combination B5+BA2.0mg/L+NAA0.1, and 1/2MS +NAA0.1mg/L IBA0.3mg/L induction result to the taking root rate is best to induce and take root.

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