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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (15): 75-77.

所属专题： 生物技术； 油料作物

• 生物技术科学 • 上一篇    下一篇

芝麻DNA高效提取及PAGE快速银染方法

吕海霞 张艳欣 王林海 张晓燕 张秀荣   

  • 收稿日期:2010-02-01 修回日期:2010-03-09 出版日期:2010-08-05 发布日期:2010-08-05
  • 基金资助:

    “十一五”国家科技支撑计划子课题;国家自然科学基金;国家自然科学基金

Methods for Efficient Extraction of Sesame Genomic DNA and Rapid Sliver Staining of PAGE

  • Received:2010-02-01 Revised:2010-03-09 Online:2010-08-05 Published:2010-08-05

PDF (PC)

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摘要：

为了快速、低成本获得高质量的芝麻基因组DNA，本实验在借鉴其他作物DNA常规提取方法基础上，简化了提取步骤，减少了试剂用量，DNA经琼脂糖电泳检测无拖尾，且OD260 /OD280在1.8～2.0之间，表明质量、纯度均较高，摸索了一套芝麻DNA高效提取方法；在常规变性聚丙烯酰胺凝胶（PAGE）银染程序基础上进行改良，减少了固定环节和试剂用量，缩短了染色和显影时间等，优化建立了芝麻PAGE快速银染方法。

关键词: 旱地红壤, 旱地红壤, 定位试验, 磷肥残效

Abstract:

In order to rapidly and economically obtain high-quality sesame genomic DNA for molecular experiments, an efficient method of DNA extraction suitable for sesame was explored, referred methods in other crops, the procedure was simplified and the cost was reduced, DNA were detected by agarose electrophoresis and no trailing phenomenon was found, their OD260/OD280 values were all between 1.8-2.0, indicating that qualities of DNA were good and high purity. Afterwards, in order to further improve the efficiency of molecular marker experiments, we established a rapid and effective silver staining procedure for polyacrylamide gels, by improving the traditional silver staining methods and controlling the staining and imaging time.