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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 48-53.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

绵羊显性白位点基因（KIT）cDNA的克隆与序列分析

张巧灵,宋德光,岳占碰,周 亮,马 雪   

  • 收稿日期:2008-07-28 修回日期:2008-09-10 出版日期:2008-11-05 发布日期:2008-11-05

Cloning and sequence analysis on KIT gene in sheeps

Zhang Qiaoling, Song Deguang, Yue Zhanpeng, Zhou Liang, Ma Xue   

  • Received:2008-07-28 Revised:2008-09-10 Online:2008-11-05 Published:2008-11-05

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摘要： 显性白位点基因座位编码一种跨膜蛋白-----肥大细胞生长因子受体，这种受体对黑色素细胞的形成、成熟及增殖迁移有重要作用。本研究克隆了绵羊KIT基因的部分编码序列，首次获得绵羊KIT基因的cDNA序列。并与其它动物相应区域作了同源性比较，结果表明：绵羊KIT基因exon11-19 cDNA长1003bp，编码含331个氨基酸残基的蛋白；蛋白质同源性比较显示，绵羊与牛、羊、猪、人、马等的同源性大于94%；通过对绵羊该蛋白进行结构域及结构分析，为进一步研究KIT基因与绵羊毛毛色的关系奠定了一定的理论基础。

Abstract: The total RNA from the skin tissure of sheeps was extracted by using Trizol reagent. Primer was designed to amplify the fragments of exon11 to exon19 in sheeps, and the fragment was obtained. It included the partial sequence of exon11 (120bp), exon12 (126bp), exon13 (90bp), exon14 (151bp), exon15 (95bp), exon16 (128bp), exon17 (123bp), exon18 (112bp), exon19 (66bp). And the deduced polypeptide was got by the translation software and it was found that the KIT gene coded 331 amino acids. The multiple sequence alignments revealed that the similarity of sheeps were above 94%. It was confirmed that the sequence obtained in this study was the KIT gene of sheeps.