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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 44-47.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

速眠新对大鼠原代肝细胞CYP3A1、CYP2E1的影响

徐丽娜,杜金梁,马玉忠   

  • 收稿日期:2008-08-15 修回日期:2008-09-09 出版日期:2008-11-05 发布日期:2008-11-05

Effects of SU-MIAN-XIN on CYP3A1 and CYP2E1 in Rat Primary Cultured Hepatocytes

Xu Lina, Du Jinliang, Ma Yuzhong   

  • Received:2008-08-15 Revised:2008-09-09 Online:2008-11-05 Published:2008-11-05

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934

摘要： 胶原酶二步灌流法获取原代大鼠肝细胞，用不同浓度的速眠新和保定宁处理，Western blot法测定肝细胞中细胞色素酶P450 3A1（CYP3A1）和细胞色素酶P4502E1 (CYP 2E1)的表达水平。结果表明：通过胶原酶灌流法，每只大鼠可获得2-4×108个肝细胞，成活率约为95%。用速眠新、保定宁处理后，肝细胞CYP3A1和CYP 2E1的表达随速眠新、保定宁浓度的增加和处理时间的延长而呈升高的趋势。原代大鼠肝细胞可用于速眠新药物代谢分子机制的研究，为其临床上合理地应用提供了理论依据。

关键词: 陆地棉, 陆地棉, 愈伤组织, 褐化, 生化代谢物

Abstract: Rat primary cultured hepatocytes were isolated by two-step collagenase digestion method and treated with gradient concentration of SU-MIAN-XIN and BAO-DING-NING, and the expression of cytochrome P450 3A1 (CYP3A1) and cytochrome P450 2E1 (CYP 2E1) were determined by Western blot. The results as follows: 2-4×108 hepatocytes per rat were obtained and the viability was about 95%. The expression of CYP3A1 and CYP 2E1 in rat hepatocytes increased gradually with treatment of SU-MIAN-XIN and BAO-DING-NING. Rat primary cultured hepatocytes can be used for molecular mechanism of SU-MIAN-XIN metabolism, it provides a foundation for its clinical application.