关键词: 水产科技期刊, 水产科技期刊, 国际化, 发展对策

Abstract: [Objectives] To compare the two bacteria typing methods as used in Enterococcus identification for swine infection. [Methods] Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitec-32 automatic identification system respectively. [Results] 34 out of 42 isolates were determined to species level by Vitec-32, among which were 10 isolates of E. faecalis, 2 of E. faecium, 22 of E. casseliflavus, whereas the other 8 failed to be identified. The 16S rRNA gene sequencing showed that all the isolates were Enterococcus species, with 12 of E. faecalis and 30 of E. faecium. In comparison, among the 10 isolates of E. faecalis by Vitec-32, 9 were verified as the same and the other 1 was identified as E. faecium by 16S rRNA gene sequencing. Both methods showed identical results with the two E. faecium isolates. All the 22 Vitec-32-determined E. casseliflavus were re-decided by 16S rRNA gene sequencing as 1 of E. faecalis and 21 of E. faecium species. The 8 unidentified isolates turned out to be E. faecalis(2) and E. faecium(6). [Conclusion] Vitec-32 results showed good consistence(75%) to that of16S rRNA gene sequencing when applied to E. faecalis identification, and significant discrepancy（93.3％） occurred in identifying the E. faecium sp isolates.