犬细小病毒VP2基因序列分析

中国农学通报 ›› 2008, Vol. 24 ›› Issue (7): 26-31.

所属专题：生物技术

犬细小病毒VP2基因序列分析

宋永奇,李玉燕,吕艳丽,韩博

收稿日期:2008-03-31 修回日期:2008-04-30 出版日期:2008-07-05 发布日期:2008-07-05

Sequence Analysis of Canine Parvovirus VP2 Gene

Song Yongqi, Li Yuyan, Lu Yanli, Han Bo

Received:2008-03-31 Revised:2008-04-30 Online:2008-07-05 Published:2008-07-05

摘要/Abstract

摘要： 2006~2007年间，从中国农业大学动物医院采集的犬细小病毒粪便样本中随机抽取20份，采用PCR技术，扩增了这20份样本的犬细小病毒VP2全基因序列。经核苷酸及推导的氨基酸序列分析发现，20个样本中，有19个样本属于CPV-2a，1个为CPV-2b，且所有病毒样本的297位均发生Ser→Ala的置换；基因型为CPV-2a病毒样本的555位均发生回归性置换(Ile→Val)。与经典的CPV-2a/b毒株比较，部分样本还出现了新位点氨基酸置换现象。其中，样本CPV/BJ005/07与CPV/BJ008/07 VP2的139位氨基酸残基由Val置换为Ile；80%（16/20）的样本在324位有Tyr→Ile的置换；样本CPV/BJ004/07和CPV/BJ050/07分别在226（Ser→Asn）与382(Arg→Lys)位发生置换。运用DNAStar软件对上述置换的氨基酸残基进行综合分析发现，除Ile-139突变意义不大外，Ile-324、Asn-226和Lys-382均处在VP2蛋白潜在的抗原表位区域，有着重要的生物学意义。

关键词: 农村信用社, 农村信用社, 盈利能力, 因素分析, 能力建设

Abstract: Using polymerase chain reaction (PCR), we amplified the complete VP2 gene of twenty canine parvovirus samples collected in small animal hospital of China Agricultural University from 2006 to 2007. sequence analysis of nucleotide and deduced amino acid residues revealed that nineteen samples were CPV-2a and one CPV-2b among the twenty canine parvovirus. All samples had an amino acid substitution of residue 297 in VP2, Ser to Ala. nineteen CPV-2a samples studied in this study had reversion substitution (Ile→Val) at residue 555 in VP2. Compared with classical CPV-2a/b isolates, some samples had new amino acid substitutions: Samples CPV/BJ005/07 and CPV/BJ008/07 at amino acid residue 139 in VP2 was Ile instead of Val; Ile-324 variants accounted to 80% (16/20). Amino acid substitutions of residue 226 (Ser→Asn) and 382 (Arg→Lys)were found in samples CPV/BJ004/07and CPV/BJ050/07, respectively. All (Ile-139, Ile-324, Asn-226, and Lys-382) except Ile-139 substitutions were all in regions of potential antigen epitopes by DNAStar software analysis, and these substitutions may have an important influence to viral biology aspect.

中图分类号:

宋永奇,李玉燕,吕艳丽,韩博. 犬细小病毒VP2基因序列分析[J]. 中国农学通报, 2008, 24(7): 26-31.

Song Yongqi, Li Yuyan, Lu Yanli, Han Bo. Sequence Analysis of Canine Parvovirus VP2 Gene[J]. Chinese Agricultural Science Bulletin, 2008, 24(7): 26-31.

参考文献

[1] 蔡宝祥.家畜传染病学[M].4版.北京:中国农业出版社,2001.
[2] Lamas-Saiz A L,Agbandje-McKenna M,Parker J SL,et al.Structural analysis of a mutation in canine parvovirus which controls antigenicity and host range[J].Virology,1996,225:65-71.
[3] Truyen U,Parrish C R.The evolution and control of parvovirus host ranges[J].J Virology,1995,6:311-317.
[4] Parrish C R,Have P,Foreyt W J,et al.The global spread and replacement of canine parvovirus strains[J].J Gen Virol,1988,69:1111-1116.
[5] Parrish C R,Aquadro C F,Strassheim M L,et al.Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus[J].J Virol,1991,65(12):6544-6552.
[6] Horiuchi M,Goto H,Ishiguro N,et al.Mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses[J].J Gen Virol,1994,75(6):1319-1328.
[7] Ikeda Y,Mochizuki M,Naito R,et al.Predominance of canine parvovirus(CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats[J].Virology,2000,278(1):13-19.
[8] Buonavoglia C,Martella V,Pratelli A,et al.Evidence for evolution of canine parvovirus type 2 in Italy[J].J Gen Virol,2001,82(12):3021-3025.
[9] Desario C, Decaro N,Campolo M,et al.Canine parvovirus infection:Which diagnostic test for virus?[J].J Virol Methods,2005,126:179-185.
[10] Wang H C,Chen W D,Lin S L,et al.Phylogenetic Analysis of Canine Parvovirus VP2 Gene in Taiwan[J].Virus Genes,2005,31(2):171-175.
[11] Pérez R, Francia L,Romero V,et al.First detection of canine parvovirus type 2c in South America[J].Vet Microbiol,2007,124:147-152.
[12] Qin Q,Loeffler I K,Li M,et al.Sequence analysis of a canine parvovirus isolated from a red panda (Ailurus fulgens) in China[J].Virus Genes,2007,34:299-302.
[13] Martella V,Decaro N,Elia G,et al.Surveillance activity for canine parvovirus in Italy[J].J Vet Med,2005,B52:312-315.
[14] Nakamura M,Tohya Y,Miyazawa T,et al.A novel antigenic variant of canine parvovirus from a Vietnamese dog[J].Arch Virol,2004,149(11):2261-2269.
[15] Desario C, Decaro N,Campolo M,et al.Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant[J].J Vet Diagn Invest,2005,17:133-138.
[16] 孙世琪,刘湘涛,郭慧琛,等.猪水泡病病毒外壳蛋白的二级结构和抗原表位预测[J].中国生物工程杂志,2006,26(5):1-6.
[17] Agbandje M,Parrish C R,Rossmann M G. The structure of parvoviruses[J].Virology,1995,6:299-309.
[18] Hueffer K,Parrish C R.Parvovirus host range,cell tropism and evolution[J].Current Opinion in Microbiology,2003,6:392-398.
[19] 吴玉章,朱锡华.一种病毒蛋白B细胞表位预测方法的建立[J].科学通报,1994,39(24):2275-2279.

犬细小病毒VP2基因序列分析

Sequence Analysis of Canine Parvovirus VP2 Gene

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