窄冠白杨组培快繁与再生体系的建立

中国农学通报 ›› 2008, Vol. 24 ›› Issue (7): 60-64.

窄冠白杨组培快繁与再生体系的建立

李际红,邢世岩,焦振,刘国兴,张友朋

收稿日期:2008-04-28 修回日期:2008-05-27 出版日期:2008-07-05 发布日期:2008-07-05

Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro

Li Jihong, Xing Shiyan, Jiao Zhen, Liu Guoxing, Zhang Youpeng

Received:2008-04-28 Revised:2008-05-27 Online:2008-07-05 Published:2008-07-05

摘要/Abstract

摘要： 以窄冠白杨为试材，对其组培快繁与再生体系的建立进行了初步的研究。研究表明，抗氧化剂聚乙烯吡咯烷酮（PVP）和抗坏血酸（Vc）能有效降低外植体的褐化；该品种外植体增殖的最适培养基为1/2MS+BA0.3mg/L+NAA0.05mg/L，平均分化率为87%，增殖系数达7.5倍，与其它处理组合均呈极显著差异。硝普钠（SNP）12mg/L极显著地促进叶片不定芽分化，其再生频率与再生芽个数分别达到96.5%与8.2。窄冠白杨适宜的生根培养基为1/2MS+IBA0.3mg/L。

关键词: 共轭亚油酸, 共轭亚油酸, 多不饱和脂肪酸, 免疫功能

Abstract: In this report, tissue culture and regeneration system from leaves in vitro have been extensively investigated utilizing Populus Leucopyramidalis as experiment materials. The results showed that the anti-oxidation materials polyvinylpyrrolidone (PVP) and ascorbic acid (Vc) decreased browning efficiently; the best medium for proliferation was 1/2 MS+ BA0.3mg/L + NAA 0.05mg/L with differentiation percentage of 87% and proliferation multiples of 7.5 respectively, and there was a great significant difference compared with other treatments. The differentiation and regeneration of adventitious shoot from leaves increased significantly in the medium MS containing BA0.3mg/L and NAA0.05mg/L supplemented with 12mg/L sodium nitroprusside (SNP) in which regeneration frequency and average number of shoots were 100% and 12 respectively. The best rooting medium was 1/2 MS+ IBA0.3mg/L .

中图分类号:

S722.37

李际红,邢世岩,焦振,刘国兴,张友朋. 窄冠白杨组培快繁与再生体系的建立[J]. 中国农学通报, 2008, 24(7): 60-64.

Li Jihong, Xing Shiyan, Jiao Zhen, Liu Guoxing, Zhang Youpeng. Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidalis in vitro[J]. Chinese Agricultural Science Bulletin, 2008, 24(7): 60-64.

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