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中国农学通报

中国农学通报 ›› 2003, Vol. 19 ›› Issue (6): 194-194.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

不同光照强度对玫瑰组织培养中初代培养物褐化的影响

杨博 , 韩振海 , 张永 , 李天红   

  • 出版日期:2003-12-25 发布日期:2003-12-25

Effect of Different Light Intensity on Browning of Rose (Rosa rugosa) Initially Cultured in vitro

Yang Bo, Han Zhenhai , Zhang Yong, Li Tianhong   

  • Online:2003-12-25 Published:2003-12-25

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摘要： 玫瑰是一种重要的观赏植物，经济价值高，但玫瑰快繁体系尚未建立。本文研究了在不同田间光照强度下，玫瑰组培初代培养过程中培养物多酚氧化酶（PPO）活性、总酚含量、褐变百分数和萌芽成活率的变化规律。结果表明：遮荫处理使玫瑰培养物的褐变百分数降低而萌芽率显著提高；正常光照强度下多酚氧化酶活性与其他遮荫处理相比并无显著变化，但总酚含量显著高于遮荫处理，二者的相关分析表明多酚氧化酶活性与组织材料的总酚含量相关性不大。

Abstract: Rose (Rosa rugosa Thunb.) is an important ornamental, but the fast propagation system hasn’t been set up yet. In this paper, we studied the effect of different light intensity on browning of rose initially cultured in vitro. Our results showed that the browning index of rose decreased and the percentage of germination increased after field treatment, especially in the light intensity of 5,333±1,751 Lx. Compared with the light intensity of 5,333±1,751 Lx and 63,078±1,651 Lx, the activity of PPO of rose under natural light intensity showed no significant difference whereas the content of phenolic substances was much higher than those treated with shaded illumination. The co-efficiency analysis demonstrated that there was no obvious relation between the activity of phenol oxidase and content of phenolic substances.