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中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 105-109.

所属专题: 生物技术 园艺

• 生物技术科学 • 上一篇    下一篇

苹果叶片蛋白质双向电泳样品制备方法的比较

曾广娟, ,李春敏,张新忠,滕云龙,董文轩   

  • 收稿日期:2008-05-04 修回日期:2008-05-26 出版日期:2008-08-08 发布日期:2008-08-08

Comparison of Protein Extraction Methods for Two-dimensional Electrophoresis in Apple Leaf

Zeng Guangjuan, , Li Chunmin, Zhang Xinzhong, Teng Yunlong, Dong Wenxuan   

  • Received:2008-05-04 Revised:2008-05-26 Online:2008-08-08 Published:2008-08-08

摘要: 为探索适用于苹果叶片蛋白质组学研究的样品制备方法,比较了三氯乙酸/丙酮沉淀法(TCA)、酚-甲醇/醋酸铵沉淀法和Tris-HCl提取法等3种总蛋白质的提取方法。制备的样品经双向电泳分离采用银染显色。结果3种方法分别得到450、374和1032个蛋白质点。认为Tris-HCl提取法为最适方法,所得图谱背景清晰,蛋白质信息量最大。

关键词: 农民创业者, 农民创业者, 社会网络, 创业资源

Abstract: In order to develop an efficient protein extraction method suitable for apple proteome analysis, three protocols for total protein extraction in apple leaf, trichloroacetic acid/acetone precipitation (TCA), phenol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared. The extracts were separated by two-dimensional electrophoresis (2-DE) followed by silver staining. The results showed that 450, 374 and 1032 protein spots were detected respectively on 2-DE gels. The Tris-HCl extraction was the most appropriate for apple leaf proteome analysis on account of the highest resolution and more informative 2-DE gels.

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