一个水稻落粒性基因SH1的SSR标记定位

中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 84-87.

所属专题：生物技术；水稻

一个水稻落粒性基因SH1的SSR标记定位

朱文银,朱镇,杨德卫,林静,赵凌,张亚东,陈涛,王才林

收稿日期:2008-04-23 修回日期:2008-05-30 出版日期:2008-08-08 发布日期:2008-08-08

Mapping of SH1, A Dominant Gene Controlling Seed Shattering Using SSR Markers in Rice (Oryza sativa L.)

Zhu Wenyin, Zhu Zhen, Yang Dewei, Lin Jing, Zhao Ling, Zhang Yadong, Chen Tao, Wang Cailin

Received:2008-04-23 Revised:2008-05-30 Online:2008-08-08 Published:2008-08-08

摘要/Abstract

摘要： 以籼稻品种93-11为轮回亲本，与粳稻品种日本晴杂交并回交的高世代分离群体为研究材料，选用104个多态性的SSR标记对水稻的落粒性基因进行定位。结果表明，在BC₄F₂群体中，6个标记的基因型来自于日本晴；在BC₄F₃定位群体中，难落粒植株数与易落粒植株数的分离比例为3:1，落粒性受1对显性基因控制，命名为SH1；分子标记与落粒性共分离分析将SH1定位在SSR标记RM5389和RM1068、RM1387之间，与3个标记的遗传距离分别为0.7cM、5.5cM和13.1cM，此结果为该基因的分子标记辅助选择奠定了基础。

关键词: 乡镇企业, 乡镇企业, 人才策略, 社会主义新农村

Abstract: In the paper, with an indica variety 93-11 as recipient and a japonica variety Nipponbare as donor, high generation backcross segregation populations were constructed. Using 104 SSR markers which display polymorphism bands between 93-11 and Nipponbare, the genetic background of BC₄F₂ and seed shattering of BC4F3 mapping population were analyzed. The results showed that in BC₄F₂ population, the genetic background analysis detected 6 markers, which distributed on rice chromosomal 1, 2 and chromosomal 5 derived from donar Nipponbare; In BC4F3 mapping population, non-shattering plants and easy shattering plants segregated in a ratio of 169:52, fitting the expected ratio of 3:1, suggesting that the seed shattering in the population was controlled by a dominant gene, named SH1; Linkage analyses suggested that the gene SH1 was linked with SSR marker RM5389, RM1068 and RM1387, the genetic distance between these markers and SH1 were 0.7cM, 5.5cM and 13.1cM, respectively. The mapping of the gene SH1 would be useful for its molecular marker-assisted selection.

中图分类号:

S41-39

朱文银,朱镇,杨德卫,林静,赵凌,张亚东,陈涛,王才林. 一个水稻落粒性基因SH1的SSR标记定位[J]. 中国农学通报, 2008, 24(8): 84-87.

Zhu Wenyin, Zhu Zhen, Yang Dewei, Lin Jing, Zhao Ling, Zhang Yadong, Chen Tao, Wang Cailin. Mapping of SH1, A Dominant Gene Controlling Seed Shattering Using SSR Markers in Rice (Oryza sativa L.)[J]. Chinese Agricultural Science Bulletin, 2008, 24(8): 84-87.

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一个水稻落粒性基因SH1的SSR标记定位

Mapping of SH1, A Dominant Gene Controlling Seed Shattering Using SSR Markers in Rice (Oryza sativa L.)

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