Abstract: The effect of the fertilization on maize photosynthetic characteristics and yield was studied on the basis of a long-term fertilization trial. the results indicated that: fertilizer significantly increased chlorophyll and photosynthetic in spike leaf of maize, dry matter accumulation as compared with the non-fertilizer treatment. The long-term combined application of organic and N fertilizer treatment increased chlorophyll and photosynthetic in spike leaf of maize obviously, compared with single N treatment, so increased the dry matter accumulation. The long-term non-fertilizer treatment reduced grains per spike ,the single N treatments can increased grains per spike, but not significantly as compared with the non-fertilizer treatment. The organic and combine application of organic and N fertilizer treatment increased grains per spike ,so the yield was higher. The yield of combined application of higher organic and higher and fertilizer treatment was highest.