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中国农学通报

中国农学通报 ›› 2003, Vol. 19 ›› Issue (6): 29-29.

所属专题： 生物技术

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狗舌草提取物对L1210细胞的体外作用研究

陈进军,,王建华,史志诚   

  • 出版日期:2003-12-25 发布日期:2003-12-25

Studies on Actions of Extract from Tephroseris Kirilowii Turcz. Holub to L1210 Cell Line in vitro

Chen Jinjun，, Wang Jianhua，Shi Zhicheng   

  • Online:2003-12-25 Published:2003-12-25

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摘要： 利用淋巴性白血病L1210细胞，以单猪屎豆碱、槲皮素和环磷酰 胺为参照，检查狗舌草60%乙醇提取物 （EX） 对L1210细胞生长情况的影响; 通过L1210细 胞台盼蓝拒染率的测定，研究EX对L1210细胞活力的影响。结果发现，一定剂量的EX、单猪 屎豆碱、槲皮素和环磷酰胺具有明显的体外抑制淋巴性白血病L1210细胞的作用，且这种作 用具有明显的剂量依赖性。其中EX和槲皮素在体外对L1210细胞的细胞毒性较小，而单猪屎 豆碱在体外对L1210细胞的细胞毒性较大。

Abstract: With a reference system of cyclophosphamide (CY), qu ercetin (QU) and monocrotaline (MO) which are in the category of compounds in Te phroseris kirilowii Turcz. Holub, the actions of the extract from T. kirilowii w ith 60% ethanol solution (EX) to murine L1210 cell were studied in vitro in the aspects of cellular culture curve and Trypan Blue resisting rate. The results sh owed that the subjects at concentrations of 1.0μg/ml（EX）、0.1μg/ml（MO）、1. 0μg/mL（QU） and 10.0μg/ml（CY）respectively gave certain effects against L121 0 cells in repression of proliferation velocity and vigor (p＜0.01).