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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (21): 64-68.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

果蔗Rar1基因反义载体的构建及遗传转化初步研究

林 生1,2,潘大仁1,2,周以飞1,2,陈观水2,张绪璋2   

  • 收稿日期:2009-07-13 修回日期:2009-08-12 出版日期:2009-11-05 发布日期:2009-11-05
  • 基金资助:

    国家自然科学基金

Construction Of Antisense Expression Vector of Rar1 fragment and Transforming into Chewing Cane

ZHOU Yi-Fei   

  • Received:2009-07-13 Revised:2009-08-12 Online:2009-11-05 Published:2009-11-05

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摘要：

摘要：本研究根据其它作物已克隆的RAR1基因的两个氨基酸保守位点设计简并引物，用果蔗cDNA进行PCR扩增，得到果蔗Rar1基因片段序列，推测出的氨基酸序列与其它作物推测出的氨基酸序列的CCCH结构域和一个锌结合域CHORD-Ⅱ具有很高的相似性，初步证明了所克隆的果蔗Rar1基因片段具有正确的序列信息。并构建了果蔗Rar1反义植物表达载体pCAM-RAR1，转化农杆菌后侵染果蔗的愈伤组织并得到初步验证，为转基因植物的获得以及果蔗Rar1基因功能鉴定奠定了基础。

关键词: 财富增长模型, 财富增长模型, 比较分析, 农村劳动力转移, 璧山县

Abstract:

Abstract: According to two conservative amino acid regions of Rar1 gene from different crops, degenerate primers were designed to amplify Rar1 fragment of chewing cane in this study. By the sequence alignment, the Rar1 fragment of chewing cane cloned from chewing cane cDNA had high similarity with the Rar1’s CCCH and CHORD-Ⅱregions of other crops. Rar1 fragment was constructed plant antisense expression vector pCAM-RAR1. The antisense gene was integrated into the genome of chewing cane callus via Agrobacterium tumefaciens EHA105 mediation. It provided a reliable base for the function s tudy on chewing cane Rar1 gene.