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中国农学通报 ›› 2010, Vol. 26 ›› Issue (1): 260-264.

所属专题: 园艺

• 农学 农业基础科学 • 上一篇    下一篇

高渗胁迫后橡胶树萌发籽苗抗旱性主成分及隶属函数分析

曾宪海,安锋,蔡明道,陈俊明,林位夫   

  • 收稿日期:2009-07-30 修回日期:2009-08-19 出版日期:2010-01-05 发布日期:2010-01-05
  • 基金资助:

    橡胶树整体水分传输及其与抗旱关系研究

Analysis of the Principal Component and the Subordinate Function on Rubber Seedling Drought Resistance under hypertonic Solution Stresses

  • Received:2009-07-30 Revised:2009-08-19 Online:2010-01-05 Published:2010-01-05

摘要:

摘要:利用NaCl、PEG6000、甘露醇模拟高渗胁迫条件,对RRIM600巴西橡胶树种子进行浸种处理7d,测定了不同浸种处理橡胶树萌发籽苗的多项生理指标,并应用主成分及隶属函数分析法对影响橡胶树苗期抗旱性的主要生理指标和不同浸种处理橡胶树萌发籽苗的抗旱性进行了分析评价。结果表明:与橡胶树苗期抗旱性关系最密切的生理指标有超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、可溶性糖(SS)、脯氨酸(PRO)、过氧化氢(H2O2)、超氧阴离子(O2-);不同浸种处理橡胶树萌发籽苗抗旱性强弱依次为:甘露醇(10%)>PEG6000(15%)>NaCl(3%)>甘露醇(15%)>PEG6000 (25%)>甘露醇(20%)>NaCl(2%)>NaCl(4%)>PEG6000 (35%)>甘露醇(20%)。

关键词: 苏云金芽胞杆菌, 苏云金芽胞杆菌, cry2Ac4基因, 诱导表达

Abstract:

Abstract:Hevea brasiliensis seeds of RRIM600 were soaked for 7days under hypertonic solution stresses of NaCl, Mannitol, PEG6000 and the seedlings which could stand the high-osmosis stress were observed to estimate the physiological indexes related to the drought resistance. The analysis of principal components and subordinate function showed that superoxide dismutase (SOD),catalase (CAT), soluble sugar (SS), praline (PRO), hydrogen peroxide (H2O2), Superoxide Free Radical (O2-) were the most important physiological indexes for drought resistance in rubber seedling, and that all the treatments under trial gave a drought resistance in the order of Mannitol (10%)>PEG6000(15%)>NaCl(3%)>Mannitol (15%)>PEG6000 (25%)>Mannitol (20%)>NaCl(2%)>NaCl(4%)>PEG6000 (35%)>Mannitol (20%)。