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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (1): 265-268.

所属专题： 园艺

• 农学 农业基础科学 • 上一篇    下一篇

铁皮石斛原球茎诱导与增殖研究

王丽萍,梁淑云   

  • 收稿日期:2009-07-29 修回日期:2009-11-05 出版日期:2010-01-05 发布日期:2010-01-05

Study on PLB Induction and propagation of Dendrobium officinale

  • Received:2009-07-29 Revised:2009-11-05 Online:2010-01-05 Published:2010-01-05

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摘要：

以铁皮石斛嫩茎为外植体，采用幼嫩茎段→原球茎诱导→原球茎增殖途径，通过单因子、双因子以及正交试验诱导原球茎。研究结果表明，外植体灭菌条件为：70%酒精浸泡3s后，0.1%升汞溶液中浸泡8min，诱导铁皮石斛原球茎最佳培养基为MS＋6-BA0.5mg/L+NAA0.5mg/L+ 2,4-D1.0 mg/L，原球茎增殖最佳培养基为MS＋6-BA3.0mg/L+NAA0.3mg/L。

关键词: 胶园, 胶园, 土壤肥力, 时空变异

Abstract:

In this paper，an experiments was carried with the bud of current year after certain treatment, followed the approach: stem→ PLB inducing→ PLB proliferating. Adopted single-factor, double-factor and orthogonal design methods, and studied the effects of the different treatments and sterilization time on the induction of PLB (protocorm-like body). The results were as following: The best sterilizing method for stem was disinfected with 70% alcohol for 3s, and then treated with 0.1% HgCl2 for 8 min. The formula of the optimal culture medium for PLB induction was the MS＋6-BA0.5 mg/L + NAA 0.5 mg/L + 2,4-D1.0 mg/L. The best medium for PLB proliferation was MS＋6-BA3.0mg/L+ NAA0.3mg/L.