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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 86-89.

• 食品 营养 检测 安全 • 上一篇    下一篇

正交试验法对黑米黑色素的微波提取工艺研究

张吉祥,白晓杰,周秋香   

  • 收稿日期:2009-09-09 修回日期:2009-09-24 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    大孔吸附树脂对中草药有效成分的提取与分离

Study on Melanin Extraction from Black Rice by Microwave and Orthogonal Test

  • Received:2009-09-09 Revised:2009-09-24 Online:2010-02-05 Published:2010-02-05

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摘要：

采用正交试验法，利用微波萃取技术，对黑米中黑色素的最佳提取工艺进行了深入研究。用分光光度法进行测定，以黑米黑色素的得率作为考查指标，对影响黑色素提取工艺的因素进行了优化研究。试验结果表明：乙醇浓度是影响黑米黑色素提取的最关键因子。优化得到黑色素的最佳提取工艺参数为：浸提剂乙醇浓度为80%、料液比1:20、微波作用功率为480W、微波作用时间为120s。在此实验条件下，黑米黑色素的得率达到3.2%，为先前文献报导的2.2倍。

关键词: 稀土, 稀土, 牛蒡低聚果糖, 草决明, ɑ-淀粉酶, 脂肪酶

Abstract:

The optimal conditions for microwave-assisted extraction of melanin from black rice were investigated by the orthogonal experiment. The content of melanin as research index in the extract was tested by spectrophotometry and the influencing factors of melanin were optimized. The results suggested that the ethanol concentration should be the statistically significant factor. The optimal parameters of the extraction process were as follows: ethanol concentration (80%), solid/liquid rate (1:20), microwave output power (480W) and microwave irradiation time (120s). Under the optimal experimental condition the extraction rate of melanin from black rice reached 3.2%, which was increased by a fctor of 2.2 compared with the former data.