Abstract: Artificial honeydew is used to imitate effect of aphid’s honeydew on the wheat yield and qualities in the three different wheat growth stages including booting stage, earring period and milking stage. The results show that agronomic characteristic, the content of the starch, wet gluten and protein are much lower than those no-damage wheat in the three stages of wheat according to the difference of concentrations of artificial honey-dew. In the booting stage, the maximal lost rate of agronomic characteristic, the content of starch, wet gluten and protein is 30.75%, 9.16%, 13.32% and 34.36%, respectively; In earring period, which is 41.79%, 8.26%, 14.83% and 39.26%. In milking stage, which is 47.21%, 9.26%, 24.01% and 44.63%. And the effects on agronomic characters and content of protein were most serious, on the content of wet gluten was less, while the content of starch was hardly affected. The effects of aphid harmfulness on yield and main nutritive qualities were different in the three different wheat stages.