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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (10): 52-54.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

家蚕抗菌肽attacin基因植物表达载体的构建

党颖慧1,杨金宏2,孔卫青2   

  • 收稿日期:2009-12-09 修回日期:2010-01-13 出版日期:2010-05-20 发布日期:2010-05-20

Construction of Plant Expression Vector with Attacin in Silkworm, Bombyx mori.

Dang Yinghui1, Yang Jinhong2, Kong Weiqing2   

  • Received:2009-12-09 Revised:2010-01-13 Online:2010-05-20 Published:2010-05-20

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摘要：

抗菌肽是昆虫抵御外界微生物侵染的主要物质。本文利用双酶切将pBI121载体上的植物启动子CaMV35S克隆到植物表达载体pCAMBIA2300上，构建重组表达载体pCAMBIA2300-CaMV35S。PCR扩增家蚕抗菌肽attacin基因编码区全长并利用T载体成功克隆（GenBank登陆号：GU244351），然后利用双酶切将attacin 基因亚克隆到pCAMBIA2300-CaMV35S上，通过PCR鉴定，成功构建了attacin基因的植物表达载体pCAMBIA2300-CaMV35S-attacin。为研究attacin基因在植物抗病性方面的应用奠定基础。

关键词: 水溶性螯合肥, 水溶性螯合肥, 常规施肥, 章丘大葱, 葱白, 维生素C, 硝酸盐含量

Abstract:

Antimicrobial peptides are the most important substance for insect in protecting themselves from infection of microorganism. In the paper, the plant-specific promoter CaMV35S from pBI121 was constructed into pCAMBIA2300, the recombinant expression vector named pCAMBIA2300-CaMV35S, attacin full-length coding region (GenBank ccession number: GU244351) as amplified by PCR from Bombyx mori and then cloned into the pMD19-T simple vector. The positive clone cutted by pairs of restriction enzymes was successfully linked with recombinant expression vector pCAMBIA2300- CaMV35S. The recombinat plasmid of attacin was identified by PCR digestion. That lay the foundation for applications of the antibacterial peptides attacin gene in disease resistance of plant.