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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (11): 366-369.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

菠萝花药愈伤组织诱导及褐变影响因素初探

蒋晶1,2,窦美安2,孙伟生2   

  • 收稿日期:2009-12-31 修回日期:2010-01-27 出版日期:2010-06-05 发布日期:2010-06-05
  • 基金资助:

    中央级公益性科研院所基本科研业务专项;广东省科技计划项目;公益性行业科研专项;广东省田园农业科技服务体系建设项目

Primary Studies on the Induction of Pineapple Anther Callus and the Factors Influence Browning

Jiang Jing1,2, Dou Mei’an2, Sun Weisheng2   

  • Received:2009-12-31 Revised:2010-01-27 Online:2010-06-05 Published:2010-06-05

PDF (PC)

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摘要：

以菠萝为试材，筛选了花药愈伤组织诱导最适激素组合，同时通过正交试验研究了基因型、取样时期及添加AgNO3等因素对菠萝花药培养过程中愈伤组织诱导及褐变的影响。结果表明：菠萝花药培养诱导愈伤组织最佳激素组合为2 mg/L 2,4-D+0.08 mg/L TDZ；正交试验三个因子对愈伤组织诱导的影响主次顺序为AgNO3>取样时期>基因型，对褐变影响主次顺序为AgNO3>基因型>取样时期，其中AgNO3最适浓度为50 mg/L。

关键词: IGF1, IGF1, 鹿茸, 生长中心细胞, 梅花鹿

Abstract:

With pineapple as the test material, the anther callus induction optimum hormone combination was selected. At the same time,by orthogonal test, effects of genotypes, sampling periods, and concentrations of AgNO3 on callus induction and browning in the process of pineapple were studied. Results showed that the optimal combination of hormone was 2 mg/L 2,4-D+0.08 mg/L TDZ. The effects of those three factors on callus induction were different, and from high to low were AgNO3, sampling period, genotype, while the effects on browning from high to low were AgNO3, genotype, sampling period, and 50 mg/L was the optimal concentration of AgNO3.