鹿茸成骨过程及其相关调控机制研究进展

doi:10.11924/j.issn.1000-6850.casb14100088

摘要/Abstract

摘要： 鹿茸是一种特殊的周期性再生的哺乳动物骨质器官。鹿茸的发生起始源于生茸区骨膜，通过膜内成骨、过渡成骨和特殊的软骨内成骨实现鹿茸的整个成骨过程。鹿茸的周期性再生及成骨过程是多种因素及信号通路综合调控下的生物学过程。笔者概述了鹿茸成骨过程的组织基础以及这一过程中相关激素、细胞因子和信号通路等的调控机制，以期为鹿茸成骨机制研究及提高鹿茸产量提供借鉴。

关键词: 夏玉米, 夏玉米, 耗水, 水分利用效率, 干旱

Abstract: Deer antlers are unique mammalian bony organs with periodic regeneration. Development of deer antlers initiate from antlerogenic periosteum, and the processes of ossification of deer antlers include intramembranous, transitional and modified endochondral ossification. Multiple regulatory factors and signaling pathways involve in the ossification processes and regenerations of antlers. This article reviewed histological reports and regulatory mechanisms of hormones, cytokines and signal paths in this process to provide a reference for the study of ossification mechanisms and the improvement of antler production.

张伟,褚文辉,李春义. 鹿茸成骨过程及其相关调控机制研究进展[J]. 中国农学通报, 2015, 31(8): 6-11.

李春义. Recent Progress on Regulatory Mechanisms of Deer Antler Ossification[J]. Chinese Agricultural Science Bulletin, 2015, 31(8): 6-11.

参考文献

[1] Li C, Suttie J M. Deer antlerogenicperiosteum: a piece of postnatally retained embryonic tissue?[J]. Cell Tissue Res,2001,204(5):375-88.
[2] Li C, Mackintosh C G, Martin S K, et al. Identification of key tissue type for antler regeneration through pedicle periosteum deletion[J]. Cell Tissue Res,2007,328(1):65-75.
[3] Li C, Yang F, Li G, et al. Antler regeneration: A dependentprocess of stem tissue primed via interaction with itsenveloping skin[J]. J Exp Zool A Ecol Genet Physiol,2007,307(2):95-105.
[4] Li C. Histogenetic aspects of deer antler development[J]. Front Biosci (Elite Ed),2013(E5):479-89.
[5] Li C, Suttie J M, Clark D E. Histological examination of antler regeneration in red deer (Cervus elaphus)[J]. Anat Rec A Discov Mol Cell Evol Biol,2005,282(2):163-74.
[6] Banks W J, Newbrey J W. Antler development as aunique modification of mammalian endochondral ossification[A]. In: R.D.Brown. Antler Development in Cervidae[C]. Kingsville, Texas: Caesar Kleberg Wildl Res Inst,1982:279-306.
[7] Bartos L, Bubenik G A, Kuzmova E. Endocrine relationships between rank-related behavior and antler growth in deer[J]. Frontiers in Bioscience E4, 2012, 4a:1111-1126.
[8] Mourik SV, Stelmasiak T. Endocrine Mechanisms and Antler Cycles in Rusa Deer, Cervus (Rusa) timorensis[A]. In: George A. Bubenik and Anthony B. Bubenik.Horns,Pronghorns and Antlers[C]. New York, USA: Springer New York,1990:416-25.
[9] Price J S, Faucheux C, Allen S. Deer antlers as a model of mammalian regeneration[J].Curr Top Develop Biol,2005,67:1-48.
[10] Rolf H, Wiese K G, Siggelkow H, et al. In vitro-studies with antler bone cells: Structure forming capacity， osteocalcin production and influence of sex steroids[J]. Osteology,2006,15(4):245-257.
[11] Li C, Littlejohn R P, Corson I D, et al. Effects of testosterone on pedicle formation and its transformation to antler in castrated male, freemartin and normal female red deer (Cervus elaphus)[J].Gen Comp Endocrinol, 2003,131(1):21-31.
[12] 高志光.梅花鹿生茸区骨膜及角柄骨膜在鹿茸生长发育中作用的研究[D].北京:中国农业科学院,2009:29-32.
[13] Bubenik G A, Brown G M, Bubenik A B, et al. Immunohistological Localization of Testosterone in the Growing Antler of the White -Tailed Deer (Odocoileus virginianus)[J]. Calc Tiss Res,1974,14(2):121-130.
[14] Bubenik G A, Miller K V, Lister A L, et al. Testosterone and Estradiol Concentrations in serum, velvet skin, and growing antler bone of male white-tailed deer[J]. J of experimental zoology,2005,303(3):186-192.
[15] Faucheux C, Nicholls B M, Allen S, et al. Recapitulation of the parathyroid hormone-related peptide–Indian hedgehog pathway in the regenerating deer antler[J].Dev Dyn,2004,231(1): 88-97.
[16] Guo B, Wang S T, Duan C C, et al. Effects of PTHrP on chondrocytes of sika deer antler[J]. Cell Tissue Res，2013,354(2):451-460.
[17] Wang S T, Gao Y J, Duan C C, et al. Effects of PTHrP on expression of MMP9 and MMP13 in sika deer antler chondrocytes[J]. Cell Biol Int,2013,37(12):1300-1307.
[18] Barling P M, Liu H, Matich J, et al. Expression of PTHrP and the PTH/PTHrP receptor in growing red deer antler[J]. Cell Biol Int, 2004,28(10):661-673.
[19] Bubenik G A, Sempere A J, Hamr J. Developing antler, a model for endocrine regulation of bone growth. Concentration gradient of T3, T4, and alkaline phosphatase in the antler, jugular, and the saphenous veins[J]. Calcif Tissue Int, 1987,41(1):38-43.
[20] Brown R D, Chao C C, Faulkner L W. Thyroxine levels and antler growth in white-tailed deer[J].Comp Biochem Physiol A Comp Physiol, 1983,75(1):71-75.
[21] 胡薇,孟星宇,田玉华,等.梅花鹿IGF1全长cDNA克隆及在鹿茸组织的表达[J].东北林业大学学报,2011,39(11):71-75.
[22] 赵振美.IGFs基因沉默对梅花鹿鹿茸软骨及间充质细胞生长发育的作用机制研究[D].武汉:华中农业大学,2013:32-38.
[23] Gu L, Mo E, Yang Z, et al. Expression and localization of insulin-like growth factor-I in four parts of the red deer antler[J]. Growth Factors,2007,25(4):264-279.
[24] Feng J Q, Chen D, Ghosh-Choudhury N, et al. Bone morphogenetic protein 2 transcripts in rapidly developing deerantler tissue contain an extended 5X non-coding region arising from adistal promoter[J]. Biochimica et Biophysica Acta,1997,1350(1):47-52.
[25] Kapanen A, Ryh?nen J, Birr E, et al. Bone morphogenetic protein 3b expressing reindeer antler[J]. J Biomed Mater Res,2002,59(1):78-83.
[26] Gyurján I Jr, Molnár A, Borsy A, et al. Gene expression dynamics in deer antler: mesenchymal differentiation toward chondrogenesis[J]. Mol Genet Genomics,2007,277(3):221-235.
[27] 杨冠,杨晓.TGF-β超家族在软骨发生、发育和维持中的作用[J].遗传,2008,30(8):953-959.
[28] 张璐,韩玉帅,郭斌,等.梅花鹿鹿茸间充质层与前成软骨层细胞的培养及SB-431542对其增殖的影响[J].中国农学通报,2011,27(11):35-38.
[29] Pan D, Zhe X, Jakkaraju S, et al. P311 induces a TGF-beta1-independent, nonfibrogenic myofibroblast phenotype[J]. J Clin Invest,2002,110(9):1349-1358.
[30] Ballock R T, Heydemann A, Wakefield L M, et al. Inhibition of the chondrocyte phenotype by retinoic acid involves the upregulation of metalloprotease genes independent of TGF-beta[J]. J Cell Physiol,1994,159(2):340-346.
[31] Allen S P, Maden M, Price J S. A Role for Retinoic Acid in Regulating the Regeneration of Deer Antlers[J]. Developmental Biology, 2002,251(2):409-423.
[32] Blum N, Begemann G. The roles of endogenous retinoid signaling in organ and appendage regeneration[J]. Cell Mol Life Sci,2013,70(20):3907-3927.
[33] Pothacharoen P, Kodchakorn K, Kongtawelert P. Characterization of chondroitin sulfate from deer tip antler and osteogenic properties[J]. Glycoconj J,2011,28(7):473-480.
[34] 刘晶莹,张帆,房金波,等.鹿茸角生成过程中的调控因子[J].特产研究,2005,27(2):61-64.
[35] 褚文辉,赵海平,杨福合,等.梅花鹿ColⅩ基因的RNAi重组慢病毒载体的构建及鉴定[J].中国农业大学学报,2009,14(4):29-34.
[36] 高志光.梅花鹿鹿茸生长及骨化与碱性磷酸酶的关系[J].吉林林学院学报,1999,15(4):233-234.
[37] Price J S, Oyajobi B O, Oreffo R O, et al. Cells cultured from the growing tip of red deer antler express alkaline phosphatase and proliferate in response to insulin-like growth factor-I[J]. J Endocrinol,1994,143(2):R9-16.
[38] Van der Eems K L, Brown R D, Gundberg C M. Circulating levels of 1,25 dihydroxyvitamin D, alkaline phosphatase, hydroxyproline, and osteocalcin associated with antler growth in white-tailed deer[J]. Acta Endocrinol (Copenh),1988,118(3):407-414.
[39] 孙浩然,郑伟,吴山力,等.梅花鹿鹿茸细胞端粒酶活性及其mRNA表达的检测[J].安徽农业科学,2011，39(2):1073-75.
[40] 刘振,赵海平,杨春,等.鹿茸再生及其分子调节机理研究进展[J].中国畜牧兽医,2013,40(2):50-53.
[41] Li C, Harper A, Puddick J, et al. Proteomes and Signalling Pathways of Antler Stem Cells[J]. Plos one,2012,7(1):1-11.
[42] 张璐.血管生成素及其受体在梅花鹿茸角中的表达与调节[D].长春:吉林大学,2012:42-48.
[43] 孙红梅.RNA干扰沉默cbfal基因对鹿茸干细胞成骨抑制作用的研究[D].北京:中国农业科学院,2010，85-92.
[44] Sun H, Yang F, Chu W, et al. Lentiviral-Mediated RNAi Knockdown of Cbfa1 GeneInhibits Endochondral Ossification of Antler Stem Cells in Micromass Culture[J].Plos one,2012,7(10):1-10.
[45] Hu W, Li T, Wu L, et al. Identification of microRNA-18a as a novel regulatorof the insulin-like growth factor-1 in the proliferation and regeneration of deer antler[J].Biotechnol Lett, 2014,36(4):703-710.
[46] Hu W, Meng X, Lu T, et al. MicroRNA1 inhibits the proliferation of Chinese sika deerderived cartilage cells by binding to the 3'-untranslated region of IGF1[J].Mol Med Rep,2013,8(2):523-528.
[47] Hu W, Li T, Hu R, et al. MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene[J]. Growth factors,2014,32(1):27-33.