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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (21): 17-23.doi: 10.11924/j.issn.1000-6850.casb2021-1173

所属专题： 生物技术； 园艺

• 农学·农业基础科学 • 上一篇    下一篇

甜菜苗期抗旱性鉴定及指标筛选

李王胜1,2(), 王雪倩1,2, 尹希龙1,2, 石杨1,2, 兴旺1,2()   

  1. 1国家甜菜种质中期库,黑龙江大学,哈尔滨 150080
    2黑龙江省普通高等学校甜菜遗传育种重点实验室/现代农业与生态环境学院,黑龙江大学,哈尔滨 150080
  • 收稿日期:2021-11-20 修回日期:2022-03-28 出版日期:2022-07-25 发布日期:2022-08-23
  • 通讯作者: 兴旺
  • 作者简介:李王胜,男,1997年出生,河北廊坊人,在读硕士研究生,研究方向：甜菜种质资源鉴定。通信地址：150000 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院,E-mail： 1595764058@qq.com。
  • 基金资助:
    国家作物种质资源库“甜菜分库运行服务”(NCGRC-2021-017);繁种与入库（圃）保存”(1921-26);农业农村部“普查收集甜菜种质资源鉴定评价与编目入库”(19210911);农业农村部“甜菜种质资源安全保存”(19211031);黑龙江省自然科学基金项目(LH2019C057);国家糖料产业技术体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102)

Drought Resistance of Sugar Beet Seedling: Identification and Index Screening

LI Wangsheng1,2(), WANG Xueqian1,2, YIN Xilong1,2, SHI Yang1,2, XING Wang1,2()   

  1. 1National Beet Germplasm Mid-term Bank, Heilongjiang University, Harbin 150080
    2Key Laboratory of Beet Genetics and Breeding, College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2021-11-20 Revised:2022-03-28 Online:2022-07-25 Published:2022-08-23
  • Contact: XING Wang

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摘要：

为筛选甜菜苗期抗旱种质资源及确定抗旱指标。本研究以国家甜菜种质中期库提供的24份甜菜种质资源为材料,采用PEG-6000模拟干旱胁迫的方法,以正常生长条件的Hoagland水培溶液(CK)为对照,对甜菜幼苗进行7天的6% PEG-6000处理,并测定地上部和地下部的表型指标。结果表明,干旱胁迫对叶鲜重、根鲜重、叶饱和鲜重和叶干重的影响较大,降幅均超过50%;且干旱胁迫后甜菜种质根冠比明显增加。聚类分析将24份甜菜种质分为四类,其中ZT000516单独为一类,是抗旱性最强的种质资源;第二类为抗旱性较强的种质分别为ZT000078、ZT000547、ZT000247;第三类为中等抗旱型种质共7份;第四类为抗旱性弱种质共13份,其中ZT001698、ZT001398为干旱高度敏感型种质资源。通过逐步回归分析筛选出叶干重和叶饱和鲜重与D值极显著相关。ZT000516为抗旱性强种质资源,ZT001698、ZT001398为干旱敏感型种质资源,叶干重和叶饱和鲜重可以作为甜菜苗期干旱胁迫快速准确地鉴定指标。

关键词: 甜菜, 苗期, 干旱胁迫, 抗旱性鉴定, 聚类分析

Abstract:

To screen the drought-resistant sugar beet germplasm resources at the seedling stage and determine the drought-resistant indexes, in this study, 24 beet germplasms provided by the National Beet Germplasm Bank were used as materials, PEG-6000 was used to simulate drought stress, and Hoagland hydroponic solution (CK) under normal growth condition was used as control. Sugar beet seedlings were treated with 6% PEG-6000 for 7 days, and the phenotype indexes of aboveground and underground parts were determined. The results showed that the effects of drought stress on leaf fresh weight, root fresh weight, leaf saturated fresh weight and leaf dry weight were significant, with a decrease rate of more than 50%, and the root-to-shoot ratio of sugar beet germplasm increased significantly after drought stress. Cluster analysis divided the 24 beet germplasms into four categories, of which, ZT000516 belonged to one category alone, which was the most drought-resistant germplasm resource. The second category had strong drought-resistance, including ZT000078, ZT000547 and ZT000247. The third category was medium drought-resistant, which had a total of 7 germplasms. The fourth category was low drought-resistant, which had 13 germplasms, of which, ZT001698 and ZT001398 were highly drought-sensitive germplasms. By stepwise regression analysis, leaf dry weight and leaf saturated fresh weight were significantly correlated with D value. ZT000516 was a germplasm with strong drought resistance, and ZT001698 and ZT001398 were drought-sensitive germplasms. Leaf dry weight and leaf saturated fresh weight could be used as indexes for rapid and accurate identification of drought stress in sugar beet seedling stage.

Key words: sugar beet, seedling stage, drought stress, identification of drought resistance, cluster analysis

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