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中国农学通报 ›› 2005, Vol. 21 ›› Issue (2): 15-15.

所属专题: 生物技术 棉花

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陆地棉多标记基因系SSR分析

孙志栋,,王学德,倪西源   

  • 出版日期:2005-02-05 发布日期:2005-02-05

Analysis of SSR on Tow Multiple-marker Lines in Upland Cotton

Sun Zhidong,,Wang Xuede, Ni Xiyuan   

  • Online:2005-02-05 Published:2005-02-05

摘要: 对陆地棉品种T582和T586两个多标记基因系及其杂交后代F1进行了SSR分析。结果表明,从50对SSR引物中筛选出6对引物具有多态性,占总引物数的12%。SSR序列位点多数呈共显性标记,少数为显性标记。SSR序列的扩增与两端设计引物有很大关系。

Abstract: Polymorphism analysis of simple sequence repeats (SSR) was carried out for parents, T582 and T586, and their F1 plants. The result of SSR analysis showed that six out of fifty pairs of primers produced DNA polymorphism, which accounts 12 % of the total. The SSR bands were more co-domination loci than domination loci. SSR-motif with DNA polymorphism was related to primers of two ends designed.