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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 33-36.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

不同品种牛IGF-I基因5′调控区序列的多态性分析

张金玉 秦立红 张国梁 赵玉民 张嘉保 赵志辉   

  • 收稿日期:2010-03-29 修回日期:2010-05-31 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    国家“863”计划项目;国家支撑计划项目

Polymorphism of IGF-I gene 5′UTR in different cattle population

  • Received:2010-03-29 Revised:2010-05-31 Online:2010-11-05 Published:2010-11-05

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摘要：

采用PCR-SSCP方法，检测134头牛IGF-I基因5′调控区的多态性。结果表明，在外显子1上游510bp处检测到一个C→T的碱基突变，有两个等位基因和三种基因型。统计结果发现，在夏洛莱牛和草原红牛群体中等位基因A占优势，但是在利木赞牛群体中却是等位基因B占优势，西门塔尔牛群体中两种等位基因的比例相对平均。AB基因型在4个群体中均为优势基因型。夏洛莱牛、西门塔尔牛、利木赞牛群体均处于Hardy-Weinberg平衡状态（P﹥0.05），而草原红牛群体处于非平衡状态（P﹤0.05）。为今后对牛的肉质性状研究打下分子生物学基础。

关键词: 农家旅馆, 农家旅馆, 准入条件, 经营标准, 质量评定

Abstract:

Abstract：The 5′UTR polymorphism of IGF-I gene was detected by PCR-SSCP in 134 cattle。The results showed that there was one single nucleotide mutation (C→T) in the sequences upstream of exon 1，there are two allelomorphic genes and three genotype。The statistics results finding that allele A was higher than others in Charolais and red steppes，allele B was higher than others in Limousin however two allelomorphic genes have identical frequency in Simmental。AB genotype was higher than others in four groups。Charolais、Simmental and Limousin groups keep a Hardy-Weinberg balanced condition but the red steppes was not。Our study settle the molecular biology foundation for the future research about carcass traits in cattle。