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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (22): 34-39.

• 生物技术科学 • 上一篇    下一篇

新型COS标记技术及其在梨属植物分子系统关系研究中的应用前景

鲍露   

  • 收稿日期:2010-04-14 修回日期:2010-06-28 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:

    安徽省砀山梨种质资源的SSR和AFLP标记分析

New Conserved Ortholog Set （COS）markers and its applications foreground in Pyrus phylogenetic research

  • Received:2010-04-14 Revised:2010-06-28 Online:2010-11-20 Published:2010-11-20

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摘要：

摘要：植物系统关系的研究以DNA序列比较分析应用最为广泛；目前，DNA序列分析的发展趋势由常用DNA片段（cpDNA, nrDNA）向低拷贝核基因（Low-copy nuclear gene, LCNG）分析发展，COS（Conserved Ortholog Set）标记就属于这一类低拷贝核基因，本文对新型COS标记的特点、COS标记的开发和应用进行综述，同时探讨其在梨属植物系统关系研究中的应用前景，从而为解决梨属植物的系统演化问题提供一种新的手段.

关键词: 自我延伸过程, 自我延伸过程, 融合PCR, 延伸时间, 融合基因

Abstract:

Abstract: DNA sequence comparison analysis is most abroad in plant phylogenetic research. At present, DNA sequence analysis is changed from common DNA fragment (cpDNA, nrDNA) to LCNG (Low-copy nuclear gene), COS (Conserved Ortholog Set) markers check LCNG site. The characteristics and development of COS markers and also their utilization and applications foreground in Pyrus are briefly reviewed in this article, which could apply a new method for resovling phylogenetic problems in Pyrus.