Abstract: Donor maize plants grown in the fields was used as experimental materials, study of technique optimization was made mainly for improving freq. of microspore survival and ELS formation. The efficient technique for the microspore culture was developed and the response of in vitro culture of isolated microspores was improved. The plantlets were regenerated from the culture. The procedure of isolated microspore culture with higher regeneration was established by the technique optimization and the platform of maize DH breeding was developed. The DH lines of maize was able to be obtained within 1 year and the field test for DH lines could be arranged in the nest year.