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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (2): 144-150.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

蝴蝶兰不同耐热性品种幼苗对高温胁迫的生理反应

杨华庚 杨重法 陈慧娟 颜速亮 陈定光   

  • 收稿日期:2010-05-31 修回日期:2010-06-17 出版日期:2011-01-14 发布日期:2011-01-14
  • 基金资助:

    海南省高等学校科学研究项目“蝴蝶兰不同品种耐热性的研究”

Response of Physiology of Phalaenopsis Cultivars with Different Heat Tolerance to High Temperature Stress at seedling stage

  • Received:2010-05-31 Revised:2010-06-17 Online:2011-01-14 Published:2011-01-14

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摘要：

摘要：分别在25℃、35℃、40℃各处理2天，测定了耐热性存在差异的两个蝴蝶兰品种幼苗的生理指标变化。结果表明，随着温度的上升，两品种的相对电导率和丙二醛含量(MDA)均不断增加，而耐热性强的品种的相对电导率和丙二醛含量增幅明显小于耐热性弱的品种；随着温度的上升，两品种的超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白含量均为先升而后降，但耐热性强的品种的SOD、POD活性、可溶性蛋白含量增幅明显大于耐热性弱的品种，而降幅明显小于耐热性弱的品种；随着温度的上升，两品种的脯氨酸和可溶性糖含量均持续增加，而耐热性强的品种的脯氨酸和可溶性糖含量增幅明显大于耐热性弱的品种。由此得出，耐热性强的品种具有较强的细胞渗透调节能力以及消除活性氧伤害的能力，是其耐热性较强的生理基础之一。

关键词: 药用野生稻, 药用野生稻, 基因组文库, 优异基因

Abstract:

Abstract:Changes of physiology index of two Phalaenopsis cultivars with different heat tolerance which treated at 25℃、35℃、40℃ for 2 day respectively were determined .The results showed that the electrolytic leakage and contents of malondialdehyde(MDA) in two Phalaenopsis cultivars with different heat tolerance were increased continuously with high temperature going up. However, the growing rate of the electrolytic leakage and contents of MDA in cultivar of heat resistance was the smaller than that in cultivar of heat sensitivity. The superoxide dismutase(SOD), peroxidase(POD) activities and solube protein content show a transient rise and then reduce with high temperature going up,but the growing rate of the SOD,POD activities and solube protein content in cultivar of heat resistance was the largest than that in cultivar of heat sensitivity,the range of decrease the SOD,POD activities and solube protein content in cultivar of heat resistance was the smaller than that in cultivar of heat sensitivity.The contents of free proline and soluble sugar were increased continuously with high temperature going up. However, the growing rate of the contents of free proline and soluble sugar in cultivar of heat resistance was the largest than that in cultivar of heat sensitivity. The above results indicated that there were better capability in maintaining osmotic adjustment and eliminating reactive oxygen species (ROS) in cultivar of heat resistance,this is one of all physiological base which cultivar of heat resistance had high heat tolerance.