欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 150-155.

所属专题： 资源与环境

• 林学 园艺 园林 • 上一篇    下一篇

基于RAPD标记的芥蓝种质资源遗传多样性分析

陈文文 刘厚诚 陈日远 宋世威 孙光闻   

  • 收稿日期:2010-06-18 修回日期:2010-08-13 出版日期:2011-04-20 发布日期:2011-04-20

Genetic Diversity Analysis of Chinese Kale Germplasm Resources by RAPD Markers

  • Received:2010-06-18 Revised:2010-08-13 Online:2011-04-20 Published:2011-04-20

PDF (PC)

1002

摘要：

调查了44份芥蓝种质的植物学性状，并利用RAPD分子标记分析了其遗传多样性。结果从150条随机引物中筛选出20个引物，20个引物共扩增出177条谱带，其中多态谱带105条，平均每个引物扩增出8.9条谱带和5.3条多态性谱，多态性比率为59.32%。基于RAPD标记，利用NTSYS-pc2.11构建了聚类树状图谱，遗传距离为0.70时，44份芥蓝资源可聚成六大类群。芥蓝种质存在着一定的遗传多样性，但原产华南而且主要产区也在华南，遗传多样性要小于芸薹属其他蔬菜。

关键词: 山东半岛滨海乡村, 山东半岛滨海乡村, 建筑聚落景观, 俚岛镇

Abstract:

The morphological characteristics of 44 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by randomly amplified polymorphic DNA (RAPD) marker. Twenty primers selected from 150 primers amplified a total of 177 bands, of which 105 were polymorphic, with an average of 7.0 bands and 3.8 polymorphic fragments per primer. The polymorphic percentage was 59.32%. The dendrogram based on RAPD data was constructed by NTSYS-pc2.1. Cluster analysis showed that 44 Chinese kale accessions could be divided into six groups at the level of D=0.70. Because Chinese kale was from south China and the main area was in south China, the genetic diversity of Chinese kale was smaller than other Brassica vegetables.

中图分类号: