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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (2): 95-98.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

番茄中WRKY2基因的克隆

李 娜 于涌鲲 赵福宽 汤世坤 孙清鹏 于同泉 路 苹   

  • 收稿日期:2010-06-28 修回日期:2010-09-09 出版日期:2011-01-14 发布日期:2011-01-14
  • 基金资助:

    北京市自然科学基金

Cloning of WRKY2 Gene in Tomato

  • Received:2010-06-28 Revised:2010-09-09 Online:2011-01-14 Published:2011-01-14

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摘要：

WRKY转录因子在植物应对生物或非生物胁迫的反应及生长和发育中起重要作用，为研究WRKY转录因子在番茄中的功能，本研究根据课题组已经克隆的番茄WRKY转录因子片段设计引物，采用RT-PCR方法，从JA诱导的番茄幼苗中，获得了608 bp的cDNA片段。序列分析表明，该序列含有WRKYGQK保守结构域，与Capsicum annuum WRKY-c序列相似性是79%，与Nicotiana tabacum NtWRKY-7序列相似性是74%，表明已经成功克隆了番茄WRKY2基因片段。

关键词: 土壤 粘土矿物 重金属污染 修复, 土壤 粘土矿物 重金属污染 修复

Abstract:

WRKY protein play an important role both in biotic and abiotic stress, also in growth and development of plant. In order to study the function of WRKY in tomato, the primers were designed according to the cloned WRKY transcription factors. The 608 bp fragment was obtained from tomato with RT-PCR method. Sequential analysis indicated that this sequence contained WRKYGQK conservative domain, and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%, respectively. The results showed that WRKY gene sequence in tomato was cloned successfully.