甘薯病毒病检测技术研究进展

摘要/Abstract

摘要： 简要概述了侵染甘薯的羽状斑驳病毒(SPFMV)、潜隐病毒(SPLV)、轻斑驳病毒(SPFMMV)、退绿矮缩病毒(SPCSV)、脉花叶病毒(SPVMV)等10余种病毒种类；着重综述了检测甘薯病毒常用的生物学、血清学、分子生物学等几种主要检测技术的原理和特点。生物学方法是依据病毒侵染后在甘薯或指示植物上的外观症状进行识别，但检测速度慢，易受季节限制；血清学技术是利用病毒外壳蛋白的抗原性，据特异性的抗体抗原免疫学反应检测病毒存在，但制备抗血清需时间长，对含量少和无蛋白外壳的病毒无法检测；分子生物学方法是以核酸为基础，通过检测病毒核酸来证实病毒的存在，该技术具有灵敏度高、特异性强、适应范围广等特点。

Abstract: in this paper, SPFMV, SPLV, SPFMMV, SPCSV and SPVMV etc. more than 10 kinds of viruses are briefly summed up. The principles and features of main detection techniques such as biology, serology, and molecular biology are introduced in detail. Biology technique is based on appearance symptom on sweet potato or Ipomoea Setosa after they are infected to identify virus. But it need long period and is apt to be limited by season. Based on coat protein(CP) of virus, serology detects virus during peculiar immunology reaction of antibody and antigen. But it can’t detect virus without CP and preparing antibody need more time. The molecular biology method is based on nucleic acid to detect virus. This technique has high sensitivity, strong veracity, wide suitability etc.

马丽,张春庆,周玉亮. 甘薯病毒病检测技术研究进展[J]. 中国农学通报, 2005, 21(2): 88-88.

Ma LiZhang Chunqing,Zhou Yuliang. Progress of Study on Sweet Potato Viruses Detection Techniques[J]. Chinese Agricultural Science Bulletin, 2005, 21(2): 88-88.

参考文献

1 Moyer JW, Kennedy GG. Purification and properities of sweet potato feather mottle virus[J].Phytopathology,1978,68:998~1004
2 Cadena-Hinojosa M A, Campbell R N. Characterization of isolates of four aphid-transmitted sweet potato viruses[J].Phyttopathology,1981,71:1086~1089
3 朱作为,薛启汉,杨永嘉,等.甘薯羽状斑驳病毒的分离和提纯[J].江苏农业科学,1994,10(1):47~49
4 李汝刚,朱笑梅,薛爱红,等.甘薯羽状斑驳病毒的研究Ⅰ甘薯羽状斑驳病毒的分离鉴定[J].植物病理学报,1992,(4):319~324
5 张维臣,马淮琴,张桂兰.甘薯病毒研究进展[J].河南农业大学学报,2000,(9):1~21
6 Moyer JW .Purification and properties of sweet potato feathery mottle virus[J].Phytopathology,1978,68:988~1004
7 Cohen J, Salomon R, Loebenstein G. An improved method for purification of Sweet potato feathery mottle virus directly from Sweet potato[J].Phytopathology,1988,78:809~811
8 孟清,张鹤龄,宋伯符,等.高效价羽状斑驳病毒抗血清的制备[J].中国病毒学,1994,9(2)151~156
9 杨崇良,路兴波,王升吉,等.甘薯羽状斑驳病毒(SPFMV)生物学性状研究[J].山东农业科学,2001,(1):26~29
10 李汝刚,薛爱红,朱笑梅,等.分泌抗甘薯羽状斑驳病毒单克隆抗体的杂交瘤细胞株构建及抗体初步研[J].生物工程学报,1992,8(4):401~403
11 Cadena-Hinojosa M A, Campbell R N. Serologic detection of feathery mottle virus strains in sweet potato and Impomoea incarnate[J].Plant Disease,1981,65:412~414
12 Aitua V,A dipala E,Carey E E,et al. The incidence of sweet potato virus disease and virus resistance of sweet potato grown in Uganda[J].Ann. Appl.Boil,1998,132:399~411
13 Okada Y, Saito A, Nishiguchi M, et al. Virus resistance in transgenic Sweet Potato expressing the coat protein gene of sweet potato feathery mottle virus[J]. Theor.Appl Genet,2001,103:743~751
14 Abad J A, Moyer J W. Detection and distribution of sweet potato feathery virus in sweet potato by in vitro-transcribed RNA probes, Membrance immunobinding assay and direct blotting[J].Phytopathology,1992,82:300~305
15 Brunt A A,Crabtree K,Pall witz M J,et al.Viruses of plants descriptions and lists from the VIDE Database[J].CAB International,1996,1484
16 Robert F, Karyeija, Jan F. Two serotypes of sweet potato feathery mottle virus in Uganda and their interaction with resistant sweet potato cultivars[J].Phytopathology,2000,90:1250~1255
17 Feo L D, None S F, Biderbost E. Etiology of sweet potato Chlorotic Dwarf Disease in Argentina[J].Plant Dis,2000,84:35~39
18 谢逸萍,马代夫,李洪民,等.甘薯病毒检测技术的改进与组培苗病毒分布特性[J].江苏农业科学,2002,(1):32~34
19 王秀丽,李广存,王泽辉,等.应用GAR-HRP进行甘薯病毒NCM-ELISA检测实验[J].山东农业科学,2002,(1):36~38
20 张振臣,李大伟.甘薯羽状斑驳病毒外壳蛋白基因在大肠杆菌中的表达及特点[J].农业生物技术学报,2000,(2):177~179
21 Aritua T,Alicai E,Adipala S,et al.Aspects of resistance to sweet potato virus disease in sweet potato[J].Ann Appl.Boil,1998,132:387~398
22 赵玖华,杨崇良,尚佑芬,等.甘薯脱毒苗的检测研究[J].山东农业科学,1995,(5):15~17
23 孟清,解峰,张鹤龄.应用免疫吸附电镜检测甘薯羽状斑驳病毒[J].内蒙古大学学报,1996,(2):245~247
24 Pio-Riberiro G,WinterS,Jarret R L,et al.Detection of sweet potato virus disease-associated closterovirus in a sweet potato accession in the United States[J].Plant Dis.1996,80:551~554
25 Ute H,Edgar M,Jelkmann W,et al.Identification of the coat protein gene of a sweet potato sunken vein closterovirus isolate from Kenya and evidence for a serolodical relationshipamong geographically diverse closterovirus isolates from sweet potato[J].Phytopathology,1996,86(7):744~750
26 邱并生,赵丰,王小凤,等.甘薯羽状斑驳病毒CDNA探针的克隆及其应用[J].微生物学报,1992,32(4):242~246
27 Kreuze J F,Kargeija R F,Gibson R W,et al.Comparison of coat protein gene sequences show that East African isolates of sweet potato feathery mottle virus from a genetically distict group[J].Arch Virol,2000,145:567~574
28 Kreuze J F,Savenkov E I,Valkonen J P T.Complete genome sequence and analysis of the subgenomic RNAS of sweet potato chlorotic sthnt virus reveal several new features for the genus crinivirus[J].Journal of virology,2002,18:9260~9270
29 王升吉,尚佑芬,杨崇良,等.甘薯羽状斑驳病毒分子生物学研究概况[J].山东农业大学学报,2001,32(4):539~543
30 Ricchmann J L,Lain S,Garcia J A.Highlights and prospects of potyvirus molecular biology[J].J.Gen Virol,1992,73:1~16
31 Chavi F,Robertson A J,Benedictus J M.Verduin survey and characterization of viruses in sweet potato from Zimbabwe[J].Plant Dis,1997,81:1115~1122
32 Shiguchi Ni,Mori M,Suzuki F,Nagata R,et al.Specific severe strains of sweet potato feathery mottle virus(SPFMV) by reverse transcription and polymerase chain reaction[J].Ann.Phytopathol Soc Japan,1995,61:119~192
33 Onuki M,Hanada K.Highly sensitive ahd simple diagnosis of virus disease of sweet potato by RT-PCR[J].Plant Protect,1996,50:102~105[in Japanese]
34 Cloinetm D,Kummert J,Lepoive P,et al.Identification of distinct potyviruses in mixed-infected sweetpotato by the polymerase chain reaction with degenerate primers[J].Phytopathology,1993,84(1):65~69
35 Nie X,Singh R P.Detection of multiple potato virus using an oligo(dt) as a common cDNA primer in multiplex RT-PCR[J].J.Virol Method,2000,86(2):79~85