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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (8): 136-139.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

金莲花组织培养和快繁体系建立的研究

杨玉芳 王玄 赵红霞 吕晋慧   

  • 收稿日期:2010-07-30 修回日期:2010-09-05 出版日期:2011-04-20 发布日期:2011-04-20

Research on Optimization System on Tissue Culture and Rapid Propagation of Trollius chinensis

  • Received:2010-07-30 Revised:2010-09-05 Online:2011-04-20 Published:2011-04-20

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摘要：

近年来，野生金莲花资源因被大量采摘而受到严重破坏，对野生金莲花进行引种驯化，并将之运用于园林绿化具有重要意义。本研究以金莲花种子为初始材料建立金莲花离体培养体系，研究不同灭菌时间对金莲花成苗的影响，同时通过比较不同培养基对金莲花增殖和生长的影响，筛选获得适宜金莲花增殖和生根培养的最佳培养基。研究结果如下：用0.1% HgCl2对金莲花种子灭菌20 min可有效抑菌；适宜金莲花增殖培养的培养基是1/2MS+1.0 mg/L 6-BA+0.1 mg/L NAA，适宜金莲花生根、壮苗培养的培养基是1/2MS或1/2MS+0.05 mg/L NAA。试验结果将为金莲花快繁和规模化生产提供依据。

关键词: 脱氧核糖核酸酶, 脱氧核糖核酸酶, 核酸内切酶, 细胞凋亡

Abstract:

Trollius chinensis has been picked dramatically in rencent years, which has enormously destroyed the wild resources of the plant. Therefore, the introduction and domestication of the wild Trollius chinensis are important for landscaping and for the protection of the wild resources .In this study, the seedlings of Trollius chinensis were used as initial explant and Trollius chinensis’s culture in vitro were established. The effect of different time of sterilization on growth of seed was studied and the optimal medium composition for rooting and proliferation was selected by comparing with the effect of different medium culture on growing of Trollius chinensis. The results showed that: the seeds of Trollius chinensis was efficiently sterilized by 0.1% HgCl2 for 20 min; the optimal medium composition for proliferation of Trollius chinensis was 1/2MS+1.0 mg/L 6-BA+0.1 mg/L NAA; the optimal medium composition for rooting and seedlings of Trollius chinensis was 1/2MS or 1/2MS+0.05 mg/L NAA. The result provided the theoretical basis for the rapid propagation and production of Trollius chinensis on a large scale.