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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 64-67.

• 林学 园艺 园林 • 上一篇    下一篇

加拿大紫荆‘森林火焰’组培快繁技术研究

李久亮 苑兆和 招雪晴 尹燕雷 朱孟涛 吴芳菲   

  • 收稿日期:2010-10-18 修回日期:2010-11-26 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    山东省农业良种产业化项目“优良园林绿化彩叶树种引进筛选”

Studies on the Techniques of Tissue Culture and Rapid Propagation for Cercis canadensis ‘Forest Pansy’

  • Received:2010-10-18 Revised:2010-11-26 Online:2011-03-20 Published:2011-03-20

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摘要：

以紫叶加拿大紫荆（Cercis canadensis ‘Forest Pansy’）当年生嫩枝为试验材料，探讨不同培养基和植物生长调节剂如6-BA、IBA、NAA、TDZ对试管苗增殖以及生根的影响。结果表明：最适增殖培养基为DKW+TDZ 0.03 mg/L+PVP 0.5 mg/L；生根培养基为1/2 MS + IBA 25 mg/L+AC 0.6 mg/L。此体系中增殖系数可达4.27，生根率73.3%。

关键词: 微波裂解, 微波裂解, 生物油, 玉米棒芯, 连续反应装置

Abstract:

Young stem sections with buds of Cercis canadensis ‘Forest Pansy’ were used as explants for experiment. The effects of culture mediums and PGRs such as 6-BA, NAA, IBA, TDZ on proliferation and rooting of test-tube plantlets were discussed. The results showed that the medium suitable for proliferation was DKW+TDZ 0.03 mg/L+PVP 0.5 mg/L, the optimum rooting medium was 1/2 MS+IBA 25 mg/L+AC 0.6 mg/L. In this system, the highest propagation coefficient was 4.27 and the rooting rate was 73.3%.

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