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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (5): 190-194.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

莪术CPD染色和45S rDNA荧光原位杂交核型分析

赵丽娟 唐菲菲 刘良科   

  • 收稿日期:2010-10-26 修回日期:2010-12-31 出版日期:2011-03-05 发布日期:2011-03-05
  • 基金资助:

    姜属植物分子细胞遗传学的研究与利用

Karyotype Analysis of Curcuma zedoaria (Christm.) Roscoe. by Using CPD Staining and FISH with 45S rDNA

  • Received:2010-10-26 Revised:2010-12-31 Online:2011-03-05 Published:2011-03-05

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摘要：

为了对莪术[Curcuma zedoaria (Christm.) Roscoe]的染色体进行识别并对该物种基因组的结构及进化进行初步研究，利用改进的火焰干燥法及荧光原位杂交技术，对莪术中期染色体的长度，着丝粒的位置及随体的数目进行分析。PI和DAPI组合（CPD）染色后和相继的45S rDNA探针荧光原位杂交结果显示，莪术具有五对45S rDNA位点，三对位于8，22，31号染色体末端的CPD带区，二对位于4，30号染色体的短臂上。第五号短臂为富含GC对的非45S rDNA位点。该实验建立了莪术的经典核型，为非整倍体，核型公式为2n＝62+1=40m+12sm+1m，其核型不对称性为2A型。

关键词: 品种比较研究, 品种比较研究

Abstract:

The aim of the study was to identify the chromosomes of Curcuma zedoaria (Christm.) Roscoe., and know something initially of the genome structure and evolution. In this study, mitotic metaphase chromosomes of Curcuma zedoaria (Christm.) Roscoe. were well prepared by using an advanced chromosome preparation method. A classic karyotype of Pennsetum was constructed according to chromosome length, centromere position, and the occurrence of NOR. By fluorescent analysis and CPD staining we found that there were five pairs of 45S rDNA signals located on different chromosomes, three pairs signals are on the NORs of the chromosomes 8, 22 and 31, the other two pairs are on the shorts arms of the chromosomes of 4 and 30, corresponding the location of CPD staining bands; the last sites of CPD staining was on the short arm of the chromosome 5. The result of this study was that Curcuma zedoaria (Christm.) Roscoe was aneuploid, and the karyotype was 2n＝62+1=40m+12sm+1m， belonging to 2A.