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中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 15-19.doi: 10.11924/j.issn.1000-6850.2013-2836

• 林学 园艺 园林 • 上一篇    下一篇

珍稀濒危物种大别山五针松花粉离体萌发的研究

韩建伟 王恩茂 张智勇 李颖岳   

  • 收稿日期:2013-10-31 修回日期:2013-12-01 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    国家林业局珍稀濒危野生动植物救护与繁育项目 “大别山五针松种子发育与遗传多样性保护研究”。

In vitro Pollen Germination Study on A Rare and Endangered Specie: Pinusdabeshanensis Cheng et Law

  • Received:2013-10-31 Revised:2013-12-01 Online:2014-05-05 Published:2014-05-05

摘要: 为了研究大别山五针松濒危的原因,并为人工杂交育种和保护该物种提供理论依据。本试验采用花粉离体萌发法,设置正交试验,研究了蔗糖、硼、钙对其萌发率及花粉管生长的影响。结果表明:蔗糖是影响大别山五针松花粉萌发和花粉管生长的关键因子,其次是硼酸,氯化钙对其影响最小;蔗糖的添加,明显促进花粉萌发,但高浓度蔗糖花粉管的生长有很大的抑制作用;适当添加硼酸可以促进花粉萌发,但不利于花粉管的生长;氯化钙对花粉萌发影响最小,不同浓度的氯化钙均可以获得较高的萌发率。综合萌发率、花粉管生长,大别山五针松花粉萌发的最适培养基为:4%蔗糖+0.05%硼酸+0.025%氯化钙。

关键词: 气候差异性, 气候差异性, 烟叶品质, 气候分型

Abstract: In order to detect the reasons why the Pinusdabeshanensis Cheng et Law is endangered and provide the evidence for the breeding and conservation. The author applied designed the orthogonal test to identify the effect of sucrose, boron, calcium on germination rate and pollen tube length using in vitro pollen germination methods. The results showed that: sucrose was the key factor in its germination and growth followed by boric acid, and calcium chloride played the least role. The ratio of pollen germination could be significantly increased by adding sucrose while it also could be limited when the sucrose was in the high level. Appropriate addition of boric acid could promote pollen germination rather than pollen tube growth. Calcium chloride had limited effect on pollen germination and it seemed that germination rate was not sensitive to the level of calcium chloride. Integrating pollen germination rate and tube growth, the optimum medium for Pinusdabeshanensis Cheng et Law pollen culture should be 4% sucrose+0.05% boric acid+0.025% calcium chloride.