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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 32-37.doi: 10.11924/j.issn.1000-6850.2008-0615

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

克伦特罗残留GC-MS检测方法的建立及对自制试纸条性能的确证

邓瑞广,张海棠,王自良,钟 华,张改平   

  • 收稿日期:2008-07-31 修回日期:2008-08-15 出版日期:2008-11-05 发布日期:2008-11-05

Development of GC-MS Method for Detecting Clenbuterol Residues and Confirmation of Self-made Strip Performance

Deng Ruiguang, Zhang Haitang, Wang Ziliang, Zhong Hua, Zhang Gaiping   

  • Received:2008-07-31 Revised:2008-08-15 Published:2008-11-05 Online:2008-11-05

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摘要： 建立了猪尿液中盐酸克伦特罗（CL）残留的GC-MS检测方法，并对自主研制的CL残留快速检测免疫金标试纸条（CL-Strip）进行了比较分析。结果表明，GC-MS的检测限为0.5ng/ml，CL-Strip的检测限为1.0ng/ml；CL-Strip与GC-MS两者的加标测定实验结果完全一致；用CL-Strip检测出的100份阴性尿样和18份阳性尿样，与GC-MS检测结果完全一致。说明CL-Strip与GC-MS同样灵敏、准确、稳定，由于CL-Strip具有简便、直观、快速、敏感、特异、准确等优点，建议在CL残留快速检测中推广应用。

Abstract: GC-MS method of detection for Clenbuterol Hydrochloride (CL) residues in swine urine was established and compared with the method of self-made strip (CL-Strip). The results showed that the detection limits of GC-MS and CL-Strip were 0.5ng/ml and 1.0ng/ml respectively. The detection results of CL added in samples were consistent completely. The detection results of 100 negative swine urine samples and 18 positive swine urine samples by CL-Strip method were agreed with GC/MS method analysis results. It can be concluded that the sensitivity, accuracy and stability of CL-Strip method were about at the same degree as GC-MS method, and CL-Strip method was recommended to be extended for its advantages in convenience, direct-viewing, rapidity, sensitivity, specificity and accuracy.