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中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 27-31.

所属专题: 生物技术 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪FcRn基因及其剪接变体序列的克隆与序列

李海霞,陈丽颖,刘 涛,范 沛,乔新安,张志强,王月影,王艳玲   

  • 收稿日期:2008-07-28 修回日期:2008-08-13 出版日期:2008-11-05 发布日期:2008-11-05

Molecular cloning and sequence analysis of Swine FcRn and its alternatively spliced variant

Li Haixia, Chen Liying, Liu Tao, Fan Pei, Qian Xin’an, Zhang Zhiqiang, Wang Yueying, Wang Yanling   

  • Received:2008-07-28 Revised:2008-08-13 Online:2008-11-05 Published:2008-11-05

摘要: 【研究目的】克隆并分析猪FcRn基因;【方法】十二指肠组织提取总RNA,利用设计的引物进行RT-PCR,PCR产物与pMD-19T载体连接后转化JM109感受态细胞,筛选阳性克隆并测序;【结果】FcRn阳性克隆两条序列在NCBI上比较,显示序列同猪FcRn(AY740682.1)同源性都为99 %。【结论】克隆了猪FcRn基因的序列和其剪接变体序列,其剪接变体序列已在GenBank 上注册(Accession.EU852582)

Abstract: 【OBJECTIVE】To clone and analysis the FcRn gene of pig.【METHOD】Total RNA was extracted from mucosal tissue of duodenum and mRNA sequence of gene were amplified by RT-PCR using two designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The sequence was analyzed to identify the recombinant plasmid.【RESULTS】Identity analysis showed that the two FcRn nucleotide sequence shared 99 % 【CONCLUSION】The porcine alternatively spliced variant of FcRn gene was successfully cloned in the present study and the sequence of spliced variant has been submitted to GenBank(Accession. EU852582)

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