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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (21): 21-24.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

肝素浓度和作用时间对绵羊精子体外获能和体外受精的影响

刘烈琴 罗桂河 孟丹娜 薛君忠 郭勇 倪和民   

  • 收稿日期:2010-03-30 修回日期:2010-09-14 出版日期:2010-11-05 发布日期:2010-11-05
  • 基金资助:

    国家“十一五”“863计划”重点项目“家畜卵泡高效诱导发育技术研究与应用”

Effects of Heparin Dosage and Sperm Capacitation Time on Ovine Sperm Capacitation and Fertilization in vitro

  • Received:2010-03-30 Revised:2010-09-14 Online:2010-11-05 Published:2010-11-05

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摘要：

摘 要：通过研究添加不同的肝素浓度(0、5、10、20、50μg/ml)及不同作用时间（0、30、45、60、90、120min）对绵羊精子体外获能、存活时间和相应体外受精的影响，为改善绵羊精子体外获能效果及研究获能的机理提供一定的参考。在获能液中添加10μg/ml肝素作用45min时，其获能率最高，顶体反应率最低（40.92％和16.59％）（P<0.05），存活时间18h，能够提高受精卵的囊胚发育；单独使用咖啡因或与10μg/ml肝素联合使用，其获能效果均不如单独使用10μg/ml肝素。说明绵羊精子获能时肝素的适宜浓度是10μg/ml，作用时间是45min，咖啡因无协同肝素促进获能的作用。

关键词: 入侵红火蚁, 入侵红火蚁, 毒液, 生物碱

Abstract:

Abstract：In order to find out some modified methods of ovine sperm capacitation in vitro, the effects of their sperm capacitation, enduring durations and fertilization after respectively adding with 0%、2%、5%、10%、20% heparin at different time (0、30、45、60、90、120min), were compared and analyzed. The results showed that ovine sperm capacitation rate was highest (40.92%), acrosome reaction was lowest(16.59%), enduring durations were longest (18h), the blastula rate was highest (20.63%), when adding with 10μg/ml heparin in the capacitation medium for 45min. The effects of capacitation when adding with caffeine or caffeine and 10μg/ml heparin, were not as good as the effects adding with 10μg/ml heparin alone. It can be recommended that it is applicable for improving ovine capacitation in vitro system with 10μg/ml heparin for 45min, caffeine don’t cooperate with heparin to improve the rate of capacitation.